大鼠、正常小鼠和加速衰老小鼠特定脑区中脑源性神经营养因子水平的年龄相关变化：与神经生长因子和神经营养素-3的比较

Age-related changes in levels of brain-derived neurotrophic factor in selected brain regions of rats, normal mice and senescence-accelerated mice: a comparison to those of nerve growth factor and neurotrophin-3.

作者信息

Katoh-Semba R, Semba R, Takeuchi I K, Kato K

机构信息

Department of Perinatology, Institute for Developmental Research, Aichi Human Service Center, Japan.

出版信息

Neurosci Res. 1998 Jul;31(3):227-34. doi: 10.1016/s0168-0102(98)00040-6.

DOI:10.1016/s0168-0102(98)00040-6

PMID:9809668

Abstract

Age-related changes in the levels of brain-derived neurotrophic factor (BDNF) in selected regions of brains from rats, normal mice and senescence-accelerated mice were compared to those of nerve growth factor (NGF) and neurotrophin-3 (NT-3). The concentration of BDNF increased with age in the rat hippocampus while it decreased in the rat cerebral cortex. The level of BDNF in the hippocampus from aged rats was about 260%, of that in the same region from young adult rats. A strong staining with antibodies specific for BDNF was observed in the hilus of the dentate gyrus in the hippocampus from aged rats. By contrast, BDNF levels were significantly lower in four brain regions from aged rats as compared to young adult rats (30, 56, 52 and 52%, lower in the septum, cerebral cortex, cerebellum and striatum, respectively). Patterns of age-related changes in the level of BDNF in the mouse hippocampus. cerebral cortex, cerebellum and olfactory bulb were similar to those in the respective regions from rats. In rats, the concentration of NGF decreased with age in the cerebral cortex but remained unchanged in the hippocampus, cerebellum and olfactory bulb. In mice, levels of NGF increased in all four brain regions from 1 to 18 months after birth. The concentrations of NT-3 increased and decreased with age in the rat cerebral cortex and cerebellum, respectively, while minimal changes were observed in the rat hippocampus and olfactory bulb as was also true in mice. In senescence-accelerated mice with memory disturbances, no marked increases in levels of NGF and BDNF in the hippocampus and in the level of NT-3 in the cerebral cortex were found. Thus, increases in levels of BDNF and NT-3 occurred in the murine hippocampus and cerebral cortex, respectively, during normal aging, but not during aging of mice with pathological changes.

摘要

比较了大鼠、正常小鼠和衰老加速小鼠大脑特定区域中脑源性神经营养因子（BDNF）水平与神经生长因子（NGF）和神经营养素-3（NT-3）水平的年龄相关变化。大鼠海马体中BDNF的浓度随年龄增加，而在大鼠大脑皮层中则降低。老年大鼠海马体中BDNF的水平约为成年年轻大鼠同一区域的260%。在老年大鼠海马体齿状回的门区观察到用BDNF特异性抗体进行的强染色。相比之下，与成年年轻大鼠相比，老年大鼠四个脑区的BDNF水平显著降低（分别在隔区、大脑皮层、小脑和纹状体中降低30%、56%、52%和52%）。小鼠海马体、大脑皮层、小脑和嗅球中BDNF水平的年龄相关变化模式与大鼠相应区域相似。在大鼠中，大脑皮层中NGF的浓度随年龄降低，但在海马体、小脑和嗅球中保持不变。在小鼠中，出生后1至18个月，所有四个脑区的NGF水平均升高。大鼠大脑皮层和小脑中NT-3的浓度分别随年龄增加和降低，而在大鼠海马体和嗅球中观察到的变化最小，小鼠也是如此。在有记忆障碍的衰老加速小鼠中，未发现海马体中NGF和BDNF水平以及大脑皮层中NT-3水平有明显升高。因此，在正常衰老过程中，小鼠海马体和大脑皮层中分别出现BDNF和NT-3水平升高，但在有病理变化的小鼠衰老过程中则没有。