Laflamme L, Brechler V, Reudelhuber T L, Gallo-Payet N, Deschepper C F
Service of Endocrinology, Faculty of Medicine, University of Sherbrooke, Canada.
Regul Pept. 1998 Oct 16;77(1-3):9-15. doi: 10.1016/s0167-0115(98)00025-1.
Angiotensin II (Ang II) increases the level of tyrosine phosphorylation of several proteins in nondifferentiated NG108-15 cells, a hybrid derived from the fusion of mouse neuroblastoma and rat glioma cells. Conversely, incubation of NG108-15 cells with an angiotensin-converting enzyme (ACE) inhibitor decreased the basal level of tyrosine phosphorylation of proteins, suggesting that locally secreted Ang II may act as an autocrine regulator. By RT-PCR, we found that nondifferentiated NG108-15 cells contained the mRNA transcript of the rat angiotensinogen, mouse renin and rat ACE genes, thus confirming that NG108-15 cells contain all the elements of a local renin-angiotensin system.
血管紧张素II（Ang II）可提高未分化的NG108-15细胞中几种蛋白质的酪氨酸磷酸化水平，NG108-15细胞是一种由小鼠神经母细胞瘤细胞和大鼠胶质瘤细胞融合而成的杂交细胞。相反，用血管紧张素转换酶（ACE）抑制剂孵育NG108-15细胞可降低蛋白质酪氨酸磷酸化的基础水平，这表明局部分泌的Ang II可能作为一种自分泌调节因子发挥作用。通过逆转录聚合酶链反应（RT-PCR），我们发现未分化的NG108-15细胞含有大鼠血管紧张素原、小鼠肾素和大鼠ACE基因的mRNA转录本，从而证实NG108-15细胞含有局部肾素-血管紧张素系统的所有成分。
