Pang P K, Kline L W
Department of Physiology, Faculty of Medicine and Oral Health Sciences, University of Alberta, Edmonton, Canada.
Regul Pept. 1998 Oct 16;77(1-3):63-7. doi: 10.1016/s0167-0115(98)00042-1.
Pituitary adenylate cyclase activating polypeptide (PACAP) was shown to relax guinea pig gallbladder strips contracted with cholecystokinin. This relaxation was mediated by PACAP interacting with VIP/PACAP receptors. PACAP was also shown to cause contraction in guinea pig gallbladder strips. The present study demonstrated that calphostin C and bisindolylmaleimide IV, both blockers of protein kinase C, significantly reduced tension, Rp-adenosine 3', 5'-cyclic monophosphatase triethylamine, a blocker of protein kinase A, had no effect on PACAP-induced tension. Nifedipine also significantly reduced the PACAP effect. The contractile effects of PACAP are mediated by protein kinase C.
垂体腺苷酸环化酶激活多肽（PACAP）可使经胆囊收缩素收缩的豚鼠胆囊条舒张。这种舒张作用是由PACAP与血管活性肠肽/ PACAP受体相互作用介导的。PACAP还可使豚鼠胆囊条收缩。本研究表明，蛋白激酶C的两种阻滞剂钙泊三醇C和双吲哚马来酰亚胺IV可显著降低张力，蛋白激酶A的阻滞剂Rp -腺苷3'，5'-环磷酸单酯三乙胺对PACAP诱导的张力无影响。硝苯地平也显著降低了PACAP的作用。PACAP的收缩作用是由蛋白激酶C介导的。
