Hafler J P, Pohl-Koppe A
Kinderklinik und Kinderpoliklinik im Dr. von Haunerschen Kinderspital, Ludwig-Maximilians-Universität, Lindwurmstr. 4, D-80337 Munich, Germany.
Eur J Med Res. 1998 Nov 17;3(11):523-6.
There is increasing evidence that the cellular immune response to Bordetella pertussis plays an important role in the immune protection. Particularly in animal models, Bordetella pertussis-specific T-cells have been shown to confer immunity. In this case report, we therefore investigated the cellular immune response to whole cell Bordetella pertussis bacteria, to the pertussis antigens filamentous hemagglutinin and pertussis toxoid defined by lymphoproliferation and cytokine secretion. Two children with whooping cough were compared to three individuals vaccinated against whooping cough with a whole cell pertussis vaccine. In contrast to the vaccinated controls, the cellular immune response to Bordetella pertussis in children with whooping cough was characterized by a strong proliferation of T cells to whole pertussis bacteria as well as to filamentous hemagglutinin and pertussis toxoid. This response was defined by a marked Th-1 type T cell response with IFN-gamma secretion to all Bordetella pertussis antigens. However, in the control individuals IFN-gamma was secreted only to whole cell Bordetella pertussis bacteria and filamentous hemagglutinin but not to pertussis toxoid. A Th-2 type cytokine response could not be detected in any condition. Our observations suggest that in the immune defense of a natural Bordetella pertussis infection, the Th-1 specific T cell response to filamentous hemagglutinin and particularly to pertussis toxoid may play a major role.
越来越多的证据表明，针对百日咳博德特氏菌的细胞免疫反应在免疫保护中发挥着重要作用。特别是在动物模型中，已证明百日咳博德特氏菌特异性T细胞具有免疫作用。因此，在本病例报告中，我们通过淋巴细胞增殖和细胞因子分泌研究了对全细胞百日咳博德特氏菌、百日咳抗原丝状血凝素和百日咳毒素的细胞免疫反应。将两名百日咳患儿与三名接种全细胞百日咳疫苗的百日咳疫苗接种者进行了比较。与接种疫苗的对照组相比，百日咳患儿对百日咳博德特氏菌的细胞免疫反应的特征是T细胞对全百日咳细菌以及丝状血凝素和百日咳毒素有强烈增殖。这种反应表现为对所有百日咳博德特氏菌抗原分泌IFN-γ的明显Th-1型T细胞反应。然而，在对照个体中，仅对全细胞百日咳博德特氏菌和丝状血凝素分泌IFN-γ，而对百日咳毒素不分泌。在任何情况下均未检测到Th-2型细胞因子反应。我们的观察结果表明，在自然百日咳博德特氏菌感染的免疫防御中，对丝状血凝素尤其是对百日咳毒素的Th-1特异性T细胞反应可能起主要作用。
