在存在肼苯哒嗪的情况下培养的成纤维细胞中DNA超螺旋的变化。
Changes in DNA supercoiling in fibroblasts cultured in the presence of hydralazine.
作者信息
Weglarz L, Koceva-Chyła A
机构信息
Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
出版信息
Biochimie. 1998 Jul;80(7):627-30. doi: 10.1016/s0300-9084(98)80025-4.
We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydralazine responded to increasing ethidium bromide concentrations in a different way than those from control cultures. That is, supercoiled loops of DNA unwound with lower concentrations of ethidium bromide sedimented less rapidly than those of control cells, indicating that hydralazine reduced the degree of DNA supercoiling. Also, nucleoids from the drug-treated cells resisted the transition from relaxed to positive supercoiling at higher concentrations of ethidium bromide. Changes in nucleoid DNA supercoiling correlated directly with the dose of hydralazine in the fibroblast culture.
我们分析了在肼苯哒嗪存在的情况下培养的小鼠成纤维细胞核DNA超螺旋的变化。从细胞中提取附着在核基质上的全部DNA，并在含有溴化乙锭的中性蔗糖密度梯度中进行沉降。用肼苯哒嗪处理的细胞中的核仁对溴化乙锭浓度增加的反应方式与对照培养细胞中的不同。也就是说，DNA的超螺旋环在较低浓度的溴化乙锭作用下解旋，其沉降速度比对照细胞的慢，这表明肼苯哒嗪降低了DNA超螺旋的程度。此外，来自药物处理细胞的核仁在较高浓度的溴化乙锭作用下抵抗从松弛超螺旋向正超螺旋的转变。核仁DNA超螺旋的变化与成纤维细胞培养中肼苯哒嗪的剂量直接相关。
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