Weglarz L, Koceva-Chyła A, Drózdz M, Jóźwiak Z
Department of Biochemistry and Chemistry, Silesian Medical Academy, Katowice, Poland.
Biochem Mol Biol Int. 1997 Oct;43(3):513-9. doi: 10.1080/15216549700204311.
The nucleoid sedimentation assay was used to study hydralazine-induced DNA structural changes and repair in the fibroblasts cultured in vitro. The drug induced a dose dependent loss in negative DNA supercoiling due to the physical breakage of the DNA. Relaxation of supercoiled DNA resulted in the nucleoids sedimenting with lower velocities than those of undamaged control cultures. Repair incubation of the cells did not cause the restoration of DNA supercoiling to control level. Unsuccessful repair of DNA damaged by hydralazine may result in maintaining the damaged DNA in the cell which could have immunologic consequences.
采用核仁沉降试验研究了体外培养的成纤维细胞中肼屈嗪诱导的DNA结构变化及修复情况。由于DNA的物理断裂，该药物导致负超螺旋DNA呈剂量依赖性损失。超螺旋DNA的松弛导致核仁沉降速度低于未受损的对照培养物。细胞的修复孵育并未使DNA超螺旋恢复到对照水平。肼屈嗪损伤的DNA修复失败可能导致受损DNA在细胞中持续存在，这可能会产生免疫后果。
