Takeda Y, Wise K S, Wang G, Grady G, Hess E V, Sharp G C, Dynan W S, Hardin J A
Medical College of Georgia Institute of Molecular Medicine and Genetics, Augusta 30912, USA.
Arthritis Rheum. 1998 Nov;41(11):2059-67. doi: 10.1002/1529-0131(199811)41:11<2059::AID-ART22>3.0.CO;2-Q.
Monoclonal antibody (mAb) F78 recognizes a heat-labile particle composed of Sm core proteins designated F78P. The objective of this study was to identify human autoantibodies recognizing the conformational structure of F78P.
Immunoblots using HeLa cell extracts without heating prior to sodium dodecyl sulfate-polyacrylamide gel electrophoresis were used to identify autoantibodies recognizing F78P. To confirm reactivities with F78P, immunoprecipitates of mAb F78 were used as a substrate for immunoblots. To identify reactivities against the F78P structure in classic anti-Sm-positive sera, autoantibodies to individual Sm core proteins were absorbed with purified U1 small nuclear RNP before immunoblotting.
We identified 2 sera that, like F78, recognized only F78P and not its component polypeptides. When classic anti-Sm antibodies were preabsorbed, the presence of F78-like, particle-specific antibodies was revealed in all of the anti-Sm-positive sera tested.
Autoantibodies against the F78P structure were commonly present in sera from patients with systemic rheumatic diseases, often in combination with4=1998 M autoantibodies.
单克隆抗体（mAb）F78识别一种由Sm核心蛋白组成的热不稳定颗粒，命名为F78P。本研究的目的是鉴定识别F78P构象结构的人类自身抗体。
使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳前未经加热的HeLa细胞提取物进行免疫印迹，以鉴定识别F78P的自身抗体。为了确认与F78P的反应性，将mAb F78的免疫沉淀物用作免疫印迹的底物。为了鉴定经典抗Sm阳性血清中针对F78P结构的反应性，在免疫印迹前，用纯化的U1小核核糖核蛋白吸附针对单个Sm核心蛋白的自身抗体。
我们鉴定出2份血清，它们与F78一样，只识别F78P，而不识别其组成多肽。当经典抗Sm抗体被预先吸附后，在所有测试的抗Sm阳性血清中都发现了F78样的颗粒特异性抗体。
针对F78P结构的自身抗体普遍存在于系统性风湿性疾病患者的血清中，通常与4 = 1998 M自身抗体同时存在。
