Tizzani L, Meacock P, Frontali L, Wésolowski-Louvel M
Centre de Génétique Moléculaire et Cellulaire, CNRS UMR 5534, Université Claude Bernard, Villeurbanne, France.
FEMS Microbiol Lett. 1998 Nov 1;168(1):25-30. doi: 10.1111/j.1574-6968.1998.tb13250.x.
The RAG3 gene of Kluyveromyces lactis, a homolog of PDC2 of Saccharomyces cerevisiae, is known to be a regulator of the pyruvate decarboxylase gene KlPDC1. We have identified new target genes for Rag3p. The RAG3 gene product was found to be required for the transcription of two genes of the biosynthetic pathway of thiamine (a cofactor of pyruvate decarboxylase). Conversely, the RAG3 gene product partially repressed the expression of the pyruvate dehydrogenase gene KlPDA1. Therefore, RAG3 may act as a general regulator in the balance of the two alternative pathways of pyruvate metabolism in yeast.
乳酸克鲁维酵母的RAG3基因是酿酒酵母PDC2的同源物，已知它是丙酮酸脱羧酶基因KlPDC1的调节因子。我们已经鉴定出Rag3p的新靶基因。发现硫胺素（丙酮酸脱羧酶的一种辅因子）生物合成途径中的两个基因转录需要RAG3基因产物。相反，RAG3基因产物部分抑制丙酮酸脱氢酶基因KlPDA1的表达。因此，RAG3可能在酵母丙酮酸代谢的两种替代途径平衡中作为一种通用调节因子发挥作用。
