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[Detection of poliovirus by digoxigenin-labeled cDNA probe prepared by PCR technique].

作者信息

Liu M T, Wang S S, Zhu T F

机构信息

Guangxi Health and Anti-epidemic Center, Nanning.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 1997 Jun;18(3):164-6.

PMID:9812466
Abstract

A study including reverse transcribed cDNAs of Sabin I, II, III virus used as templates and digoxigenin-labeled dUTP added to the reaction solution was carried out. After amplification, digoxigenin-labeled poliovirus cDNA probe was prepared by this PCR technique. Results showed that the direct preparation of digoxigenin-labeled poliovirus cDNA probe by PCR is a convenient and rapid method with a high labeling rate. Compared with neutralization test, the probe has the advantages of more rapid, more sensitive and more specific for poliovirus typing.

摘要

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