石杉碱甲对小鼠体外神经肌肉传递的促进作用。
Facilitatory effect of huperzine-A on mouse neuromuscular transmission in vitro.
作者信息
Lin J H, Hu G Y, Tang X C
机构信息
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
出版信息
Zhongguo Yao Li Xue Bao. 1996 Jul;17(4):299-301.
AIM
To study the effects of huperzine-A on neuromuscular junction transmission in mouse.
METHODS
The isolated mouse phrenic nervehemidiaphragm preparations were used with the conventional intracellular recording technique. The spontaneous electrical activities of cholinergic nerve terminals (miniature end-plate potentials, MEPP) were recorded.
RESULTS
Huperzine-A, tacrine, and E2020 at the concentrations of 0.05-1 mumol.L-1 increased the amplitude, mean rise time, and half decay time of MEPP in a concentration-dependent manner. Their potencies were E2020 > huperzine-A > tacrine.
CONCLUSION
The anticholinesterase action of huperzine-A in cholinergic synapses is stronger than that of tacrine.
目的
研究石杉碱甲对小鼠神经肌肉接头传递的影响。
方法
采用常规细胞内记录技术，制备离体小鼠膈神经 - 半膈肌标本。记录胆碱能神经末梢的自发电活动（微小终板电位，MEPP）。
结果
浓度为0.05 - 1 μmol·L-1的石杉碱甲、他克林和E2020均以浓度依赖的方式增加MEPP的幅度、平均上升时间和半衰减时间。它们的效能为E2020 > 石杉碱甲 > 他克林。
结论
石杉碱甲在胆碱能突触中的抗胆碱酯酶作用强于他克林。
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