Zhang G, Feng Z, Zhang H, Li D, Fan Q
Department of Medical Molecular Biology, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1997;17(3):129-31. doi: 10.1007/BF02888285.
On the basis of preparation of anti-metastatic recombinant FN polypeptides, CH50 and CH56, we further studied the function of these polypeptides. The capacity of CH50 binding with melanoma cells (ED50 30 mM) was higher than that of CH56 (ED50 45 mM). Both of the polypeptides could significantly suppress the binding of melanoma B16 cells to laminin. There was no significant difference in the inhibitory effect between two polypeptides. In the experimental metastasis of melanoma cells, both of CH50 and CH56 could significantly inhibit the metastasis of the tumor cells, and reduce the number of lung metastasis by about 80%. Our results suggest that III-11 and ED-A repeats influenced, to some extent, the binding capacity of bifunctional-domain polypeptide to cells, but did not affect the inhibition of the polypeptide on the metastasis of melanoma cells. The presence and connection of cell I and Hep II domains are the elements which determine the ability of recombinant FN polypeptides to inhibit the metastasis of tumor cells.
在制备抗转移重组纤连蛋白多肽CH50和CH56的基础上，我们进一步研究了这些多肽的功能。CH50与黑色素瘤细胞结合的能力（半数有效剂量为30 mM）高于CH56（半数有效剂量为45 mM）。这两种多肽都能显著抑制黑色素瘤B16细胞与层粘连蛋白的结合。两种多肽的抑制效果没有显著差异。在黑色素瘤细胞的实验性转移中，CH50和CH56都能显著抑制肿瘤细胞的转移，并使肺转移数量减少约80%。我们的结果表明，III-11和ED-A重复序列在一定程度上影响了双功能域多肽与细胞的结合能力，但不影响该多肽对黑色素瘤细胞转移的抑制作用。细胞I和Hep II结构域的存在及连接是决定重组纤连蛋白多肽抑制肿瘤细胞转移能力的因素。
