Liu C R, He F C, Wu Z Z
Institute of Developmental Biology, School of Life Sciences, Shandong University, Jinan.
Sheng Li Xue Bao. 1997 Jun;49(3):255-60.
By using Y chromosome specific sex-determining region (Sry) as a new cytogenetic marker and PCR technique, the characteristics of proliferation and differentiation of hematopoietic stem cells in mice were studied. Bone marrow cells from male mice were injected into lethally-irradiated female mice, PCR results indicated that all of the CFU-S were originated from donor. It was found that CFU-S was able to proliferate and differentiate into various hematopoietic cells in vivo during its transplantation. Whereas the fibroblasts within donor CFU-S and the fibroblasts from bone marrow in recipient mouse reestablished by donor CFU-S were shown to be originated from the recipient. The above data demonstrated that CFC-S from bone marrow in mice possessed the characteristics of hematopoietic stem cells but was not prone to differentiate into fibroblasts.
通过使用Y染色体特异性性别决定区（Sry）作为一种新的细胞遗传学标记和PCR技术，研究了小鼠造血干细胞的增殖和分化特性。将雄性小鼠的骨髓细胞注射到经致死剂量照射的雌性小鼠体内，PCR结果表明所有的脾集落形成单位（CFU-S）均来自供体。研究发现，CFU-S在移植过程中能够在体内增殖并分化为各种造血细胞。而供体CFU-S中的成纤维细胞以及由供体CFU-S重建的受体小鼠骨髓中的成纤维细胞显示来自受体。上述数据表明，小鼠骨髓中的集落形成细胞（CFC-S）具有造血干细胞的特性，但不易分化为成纤维细胞。
