Arienti G, Carlini E, Saccardi C, Palmerini C A
Istituto di Biochimica e Chimica Medica, Perugia, Italy.
Biochim Biophys Acta. 1998 Sep 16;1425(1):36-40. doi: 10.1016/s0304-4165(98)00047-6.
Prostasomes are membranous vesicles (150-200 nm diameter) present in human semen. They are secreted by the prostate gland and contain large amounts of cholesterol, sphingomyelin and Ca2+. In addition, some of their proteins are enzymes. Prostasomes enhance the motility of ejaculated sperm and are involved in a number of biological functions. In a previous work, we found that prostasome can fuse to spermatozoa at slightly acidic pH values, as demonstrated by the transfer of the lipophilic octadecylrhodamine probe. In this paper, we study the interactions of two leukocyte populations (polymorphonuclear and mononuclear) with prostasomes and find a pH-dependent adhesion (revealed by microscopic observation), but no fusion. These phenomena may be relevant for the functions of leukocytes in human reproduction.
前列腺小体是存在于人类精液中的膜性小泡（直径150 - 200纳米）。它们由前列腺分泌，含有大量胆固醇、鞘磷脂和钙离子。此外，它们的一些蛋白质是酶。前列腺小体可增强射出精子的活力，并参与多种生物学功能。在之前的一项研究中，我们发现，如亲脂性十八烷基罗丹明探针的转移所示，前列腺小体在略呈酸性的pH值下可与精子融合。在本文中，我们研究了两种白细胞群体（多形核白细胞和单核白细胞）与前列腺小体的相互作用，发现了一种pH依赖性黏附（通过显微镜观察揭示），但未发现融合现象。这些现象可能与白细胞在人类生殖中的功能有关。
