Kalichman M W, Dines K C, Bobik M, Mizisin A P
Department of Pathology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0612, USA.
Brain Res. 1998 Nov 9;810(1-2):130-7. doi: 10.1016/s0006-8993(98)00898-1.
In an initial study, the effects of galactose intoxication on nerve laser Doppler blood flow (NLDF) and nerve conduction velocity (NCV) were assessed after 1-16 weeks of galactose feeding in pentobarbital-anesthetized rats. NLDF was not significantly changed at any time point. NCV was significantly reduced after 16, but not 1 or 4, weeks of galactose feeding. In a second study, NLDF was not significantly changed by 4 weeks of galactose intoxication, but streptozotocin-diabetic NLDF was significantly reduced compared to both control (P<0.001) and galactose-intoxicated rats (P<0.05). Compared to control animals, sciatic motor NCV was significantly (P<0.001) reduced in the galactose group, while sciatic and saphenous sensory NCVs were not significantly changed. In the streptozotocin-diabetic rats, motor and sensory NCVs were all significantly reduced (P<0.001). In contrast to the NCV findings, mean caliber of myelinated axons in both the saphenous and sciatic nerves was reduced in galactose-intoxicated, but not streptozotocin-diabetic rats. The observed sequence of changes associated with these two models of diabetic neuropathy is not consistent with the proposed roles of ischemia and axonal dwindling in the reported nerve conduction deficits.
在一项初步研究中，对戊巴比妥麻醉的大鼠喂食半乳糖1 - 16周后，评估半乳糖中毒对神经激光多普勒血流（NLDF）和神经传导速度（NCV）的影响。在任何时间点，NLDF均无显著变化。喂食半乳糖16周后NCV显著降低，但喂食1周或4周后未出现显著降低。在第二项研究中，半乳糖中毒4周后NLDF无显著变化，但与对照组（P<0.001）和半乳糖中毒大鼠组（P<0.05）相比，链脲佐菌素诱导的糖尿病大鼠的NLDF显著降低。与对照动物相比，半乳糖组大鼠的坐骨神经运动NCV显著降低（P<0.001），而坐骨神经和隐神经的感觉NCV无显著变化。在链脲佐菌素诱导的糖尿病大鼠中，运动和感觉NCV均显著降低（P<0.001）。与NCV结果相反，半乳糖中毒大鼠的隐神经和坐骨神经中有髓轴突的平均直径减小，而链脲佐菌素诱导的糖尿病大鼠则没有。观察到的与这两种糖尿病神经病变模型相关的变化顺序与报道的神经传导缺陷中缺血和轴突萎缩的作用不一致。
