Alkondon M, Pereira E F, Albuquerque E X
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 655 W. Baltimore St., Baltimore, MD 21201, USA.
Brain Res. 1998 Nov 9;810(1-2):257-63. doi: 10.1016/s0006-8993(98)00880-4.
This study demonstrates for the first time that alpha7 nicotinic receptors (nAChRs) mediate fast synaptic transmission in conventional hippocampal slices. In the presence of antagonists of muscarinic, AMPA, NMDA, GABAA, ATP, and 5-HT3 receptors, spontaneous and evoked postsynaptic currents (PSCs) recorded from CA1 interneurons were blocked by the alpha7 nAChR antagonists methyllycaconitine and alpha-bungarotoxin and by a desensitizing concentration of the alpha7 nAChR agonist choline. Spontaneous nicotinic PSCs were also accompanied by Na+ transients, indicating that alpha7 nAChR-mediated transmission serves as an excitatory signal to the CA1 interneurons in the hippocampus.
本研究首次证明，α7烟碱型受体（nAChRs）介导传统海马脑片中的快速突触传递。在毒蕈碱、AMPA、NMDA、GABAA、ATP和5-HT3受体拮抗剂存在的情况下，从CA1中间神经元记录的自发和诱发突触后电流（PSC）被α7 nAChR拮抗剂甲基lycaconitine和α-银环蛇毒素以及α7 nAChR激动剂胆碱的脱敏浓度所阻断。自发烟碱型PSC也伴随着Na+瞬变，表明α7 nAChR介导的传递作为海马CA1中间神经元的兴奋性信号。
