Rodríguez M M, Bisset J A, Mastrapa L, Díaz C
Instituto de Medicina Tropical Pedro Kourí.
Rev Cubana Med Trop. 1995;47(3):154-60.
The mechanisms of resistance in Culex quinquefasciatus from 6 municipalities of Havana City were studied in order to determine their genetic frequency. Increased esterases and altered acetylcholinesterase are still being the major resistance mechanisms in Havana City. The mechanisms of esterases is of high frequency, followed by altered acetylcholinestenase. Resistance to cholorpirifos was found for the first time in Culex quinquefasciatus, while resistance of malathion and carbamate propoxur is maintained, and deterioration to pyrethroid susceptibility is being detected. The synergistic effect of S.S.S. trubutyl phosphotritiade (DEF) and piperonil butoxide (PB) was analysed, and it was proven that esterases enzymes and multifunction oxidases are involved in the resistance to pyrethroids. By electrophoresis in polyerylamide gel, it was determined that the combination of esterases that seems to be associated with the resistance to perythroids is B1-A6-B6, which appeared with the greatest percentage. Valves of genetic frequency in the 6 municipalities were found to be high, for both esterase genes and acetylcholinesterase genes (Ache).
为了确定哈瓦那市6个市的致倦库蚊的抗性机制及其基因频率，对其进行了研究。酯酶增加和乙酰胆碱酯酶改变仍是哈瓦那市主要的抗性机制。酯酶机制频率较高，其次是改变的乙酰胆碱酯酶。在致倦库蚊中首次发现对毒死蜱有抗性，而对马拉硫磷和氨基甲酸酯类残杀威的抗性依然存在，并且检测到对拟除虫菊酯的敏感性在下降。分析了三丁基磷酸三硫酯（DEF）和胡椒基丁醚（PB）的增效作用，结果证明酯酶和多功能氧化酶参与了对拟除虫菊酯的抗性。通过聚丙烯酰胺凝胶电泳确定，似乎与对拟除虫菊酯抗性相关的酯酶组合是B1 - A6 - B6，其出现的百分比最高。发现酯酶基因和乙酰胆碱酯酶基因（Ache）在这6个市的基因频率阀值都很高。
