蛋白质和磷脂酰丝氨酸脂质体表面磷酸钙晶体的成核与生长。

The nucleation and growth of calcium phosphate crystals at protein and phosphatidylserine liposome surfaces.

作者信息

Nancollas G H, Tsortos A, Zieba A

机构信息

Department of Chemistry, State University of New York at Buffalo, Amherst, USA.

出版信息

Scanning Microsc. 1996;10(2):499-507; discussion 508.

PMID:9813627

Abstract

The kinetics of calcium phosphate crystal growth at the surfaces of proteins and phospholipids has been investigated using free drift and constant composition methods in supersaturated calcium phosphate solutions (relative supersaturations: with respect to hydroxyapatite, HAP, sigma HAP = 15.0, and with respect to octacalcium phosphate, OCP, sigma OCP = 1.9). Fibrinogen and collagen molecules adsorbed at hydrophobic surfaces as well as uncross-linked collagen fibrils induce ion binding and subsequent nucleation of calcium phosphate. The formation of OCP on phosphatidylserine vesicles introduced to highly supersaturated calcium phosphate solutions probably involves the interaction of the calcium ions with the ionized carboxylic groups of the phospholipid.

摘要

在过饱和磷酸钙溶液（相对于羟基磷灰石，HAP，σHAP = 15.0；相对于磷酸八钙，OCP，σOCP = 1.9）中，使用自由漂移法和恒定组成法研究了磷酸钙晶体在蛋白质和磷脂表面的生长动力学。吸附在疏水表面的纤维蛋白原和胶原蛋白分子以及未交联的胶原纤维会诱导离子结合以及随后磷酸钙的成核。引入到高度过饱和磷酸钙溶液中的磷脂酰丝氨酸囊泡上OCP的形成可能涉及钙离子与磷脂的离子化羧基的相互作用。