用各种癌基因转染的SHOK细胞对195mPt标记的顺二氯二氨铂（II）的抗性。
Resistance to 195mPt-radiolabeled cis-diaminedichloroplatinum (II) of SHOK cells transfected with various oncogenes.
作者信息
Kinashi Y, Akaboshi M, Masunaga S, Ono K, Watanabe M
机构信息
Research Reactor Institute, Kyoto University, Osaka, Japan.
出版信息
Radiat Med. 1998 Jul-Aug;16(4):233-7.
SHOK (Syrian hamster Osaka-Kanazawa) cells were transfected with activated oncogenes (v-mos, c-myc, N-ras, H-ras, K-ras). These oncogene-transfected cells were treated with 195mPt-cis-diaminedichloroplatinum (II) (CDDP). Clonogenic cell survival assay showed that oncogene-transfected cells exhibited 1.3-4.8 fold (v-mos; 2.4, c-myc; 3.6, N-ras; 1.3, H-ras; 4.8, K-ras; 2.3) increased resistance to cisplatin compared with the parental SHOK cells. The distribution of DNA, RNA, and protein fractions of treated cells was determined using the method of Schneider. The CDDP concentrations binding to DNA, RNA, and protein were measured by counting 195mPt-radioactivity. CDDP uptake was decreased in these oncogene-transfected cells. However, the range of reduction of CDDP uptake in DNA was smaller than the resistance range demonstrated in the cell killing efficiency.
将活化的癌基因（v-mos、c-myc、N-ras、H-ras、K-ras）转染叙利亚仓鼠大阪-金泽（SHOK）细胞。用195mPt-顺二氨基二氯铂（II）（CDDP）处理这些转染癌基因的细胞。克隆形成细胞存活试验表明，与亲代SHOK细胞相比，转染癌基因的细胞对顺铂的抗性增加了1.3至4.8倍（v-mos；2.4，c-myc；3.6，N-ras；1.3，H-ras；4.8，K-ras；2.3）。使用施奈德方法测定处理细胞的DNA、RNA和蛋白质组分的分布。通过计数195mPt放射性来测量与DNA、RNA和蛋白质结合的CDDP浓度。这些转染癌基因的细胞中CDDP摄取减少。然而，DNA中CDDP摄取的减少范围小于细胞杀伤效率中显示的抗性范围。
Zotero 插件