Suehara N, Mizumoto K, Tanaka M, Niiyama H, Yokohata K, Tominaga Y, Shimura H, Muta T, Hamasaki N
Departments of Surgery, Kyushu University Faculty of Medicine, Fukuoka 812-82, Japan.
Clin Cancer Res. 1997 Dec;3(12 Pt 1):2479-83.
Telomerase activity was measured in pancreatic juice obtained by endoscopic retrograde pancreatography from 34 patients (12 with ductal carcinoma, 12 with pancreatic adenoma, and 10 with pancreatitis). The activity in pancreatic juice was expressed as the number of cells of a human pancreatic cancer cell line, MIA PaCa-2, that exhibit an activity equal to that expressed in 1 microg of protein from pancreatic juice. A telomerase ladder was detected in the pancreatic juice obtained from a majority of the patients with ductal adenocarcinoma. The median value of relative telomerase activity in the carcinoma samples was 9.38 (25th percentile, 3.14; 75th percentile, 95.8), a value significantly higher than that derived from patients with either pancreatitis or pancreatic adenoma (P < 0.0001). When a threshold value of relative telomerase activity of 3.00 was used, 75% (9 of 12) of the samples obtained from patients with ductal carcinoma were positive. We conclude that telomerase activity in pancreatic juice differentiates adenocarcinoma from adenoma and pancreatitis and may serve as a useful diagnostic tool.
通过内镜逆行胰胆管造影术从34例患者（12例导管癌、12例胰腺腺瘤和10例胰腺炎患者）获取胰液，检测其中的端粒酶活性。胰液中的活性以人胰腺癌细胞系MIA PaCa-2的细胞数量表示，该细胞系表现出的活性与1微克胰液蛋白中所表达的活性相当。在大多数导管腺癌患者获取的胰液中检测到了端粒酶梯状条带。癌样本中相对端粒酶活性的中位数为9.38（第25百分位数为3.14；第75百分位数为95.8），该值显著高于胰腺炎或胰腺腺瘤患者的值（P < 0.0001）。当相对端粒酶活性阈值为3.00时，从导管癌患者获取的样本中有75%（12例中的9例）呈阳性。我们得出结论，胰液中的端粒酶活性可将腺癌与腺瘤和胰腺炎区分开来，可能是一种有用的诊断工具。
