Ralhan R, Kaur J
Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, India.
Clin Cancer Res. 1995 Oct;1(10):1217-22.
Transformed cells differ from normal cells in their pattern of heat shock protein expression. Here, we report differential expression of a Mr 70,000 heat shock protein (HSP70) in squamous cell carcinomas of the uterine cervix compared to normal or premalignant uterine cervix. Expression of HSP70 was measured using a mouse mAb against HSP70 by an ELISA. A significant increase in the level of expression of HSP70 was observed in fresh tumor cells as compared to normal or dysplastic ones (P < 0.001). The induction of HSP70 in tumor cells subjected to hyperthermia was less than that in normal or premalignant cells. Strong cytoplasmic and nuclear immunostaining was observed in cancerous tissues using anti-HSP70 mAb, biotinylated rabbit antimouse immunoglobulins, avidin combined in vitro with biotinylated horseradish peroxidase, and diaminobenzidine tetrachloride. HSP70 immunostaining was not detected in normal or dysplastic tissue sections. The results were confirmed by immunoprecipitation using anti-HSP70 mAb. These results are the first demonstration of overexpression of HSP70 in squamous cell carcinomas of the uterine cervix as compared to that in dysplastic or normal uterine cervix cells. We conclude that tumor cells differ from normal cells in their pattern of HSP70 expression, and increased levels of HSP70 correlate with an increase in tumor size.
转化细胞在热休克蛋白表达模式上与正常细胞不同。在此，我们报告与正常或癌前子宫颈相比，子宫颈鳞状细胞癌中一种分子量为70,000的热休克蛋白（HSP70）表达存在差异。使用抗HSP70的小鼠单克隆抗体通过酶联免疫吸附测定法（ELISA）检测HSP70的表达。与正常或发育异常的细胞相比，新鲜肿瘤细胞中HSP70的表达水平显著增加（P < 0.001）。热疗处理后的肿瘤细胞中HSP70的诱导水平低于正常或癌前细胞。使用抗HSP70单克隆抗体、生物素化兔抗小鼠免疫球蛋白、体外与生物素化辣根过氧化物酶结合的抗生物素蛋白以及四氯化二氨基联苯胺，在癌组织中观察到强细胞质和细胞核免疫染色。在正常或发育异常的组织切片中未检测到HSP70免疫染色。使用抗HSP7抗体的免疫沉淀法证实了这些结果。这些结果首次证明与发育异常或正常子宫颈细胞相比，子宫颈鳞状细胞癌中存在HSP70的过表达。我们得出结论，肿瘤细胞在HSP70表达模式上与正常细胞不同，且HSP70水平升高与肿瘤大小增加相关。
