Toi M, Inada K, Hoshina S, Suzuki H, Kondo S, Tominaga T
Department of Surgery, Tokyo Metropolitan Komagome Hospital, 3-18-22, Honkomagome, Bunkyo-ku, Tokyo, 113, Japan.
Clin Cancer Res. 1995 Sep;1(9):961-4.
Vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF) are known to be angiogenic growth factors in vitro and in vivo. In this study, we have investigated the relationship between VEGF expression and PD-ECGF expression in human breast cancer tissues using immunocytochemical methods. Of 152 primary breast cancers, 84 (55.3%) and 71 (46.7%) were positive for VEGF and PD-ECGF, respectively. Fifty-three (63. 1%) of 84 VEGF-positive tumors had a PD-ECGF-positive phenotype, whereas only 18 (26.5%) of 68 VEGF-negative tumors had a PD-ECGF-positive phenotype. There was a significant correlation between the VEGF expression and PD-ECGF expression (P < 0.01). As a single factor, VEGF expression and PD-ECGF expression were significantly associated with an increase in the microvessel density assessed by the immunocytochemical analysis using antifactor VIII-related antigen mAb. Interestingly, in addition, of 53 tumors with more than 100 microvessel counts/1 mm2, 40 (75.5%) had both VEGF- and PD-ECGF-positive phenotypes. It was found that VEGF and PD-ECGF were frequently coexpressed in highly vascularized tumors with high microvessel counts. It is suggested that VEGF and PD-ECGF might cooperatively function in the neovascularization of human breast cancer.
血管内皮生长因子（VEGF）和血小板衍生的内皮细胞生长因子（PD - ECGF）在体内外均被认为是血管生成生长因子。在本研究中，我们使用免疫细胞化学方法研究了人乳腺癌组织中VEGF表达与PD - ECGF表达之间的关系。在152例原发性乳腺癌中，VEGF和PD - ECGF阳性分别为84例（55.3%）和71例（46.7%）。84例VEGF阳性肿瘤中有53例（63.1%）具有PD - ECGF阳性表型，而68例VEGF阴性肿瘤中只有18例（26.5%）具有PD - ECGF阳性表型。VEGF表达与PD - ECGF表达之间存在显著相关性（P < 0.01）。作为单一因素，VEGF表达和PD - ECGF表达与使用抗因子VIII相关抗原单克隆抗体通过免疫细胞化学分析评估的微血管密度增加显著相关。此外，有趣的是，在53例微血管计数超过100个/1mm² 的肿瘤中，40例（75.5%）具有VEGF和PD - ECGF双阳性表型。研究发现，VEGF和PD - ECGF在微血管计数高的高度血管化肿瘤中经常共同表达。提示VEGF和PD - ECGF可能在人乳腺癌的新生血管形成中协同发挥作用。
