Cerutti J, Trapasso F, Battaglia C, Zhang L, Martelli M L, Visconti R, Berlingieri M T, Fagin J A, Santoro M, Fusco A
Dipartimento di Biologia e Patologia Cellulare e Molecolare, c/o Centro di Endocrinologia ed Oncologia Sperimentale del CNR, Facoltà di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli "Federico II," via Pansini, 5, 80131 Napoli, Italy.
Clin Cancer Res. 1996 Jan;2(1):119-26.
Although elevated c-myc expression seems to be related to an unfavorable prognosis of human thyroid neoplasias, the role of c-myc overexpression in the process of thyroid carcinogenesis is still unknown. We analyzed c-myc expression in 7 human thyroid carcinoma cell lines, originating from different histotypes, and in 50 fresh thyroid tumors and found a higher level of c-myc mRNA in all the thyroid carcinoma cell lines and in several fresh thyroid tumors compared with normal thyroid. The highest increases occurred in the most malignant cell lines and in undifferentiated human thyroid carcinomas. The block of c-MYC protein synthesis with myc-specific antisense oligonucleotides reduced the growth rate of the thyroid carcinoma cell lines significantly. Our results indicate that c-myc overexpression plays a critical role in the growth of thyroid cancer cells, which supports the hypothesis that the myc proto-oncogene might be involved in the neoplastic progression of thyroid carcinogenesis.
尽管c-myc表达升高似乎与人类甲状腺肿瘤的不良预后相关，但c-myc过表达在甲状腺癌发生过程中的作用仍不清楚。我们分析了来自不同组织类型的7种人甲状腺癌细胞系以及50个新鲜甲状腺肿瘤中的c-myc表达，发现与正常甲状腺相比，所有甲状腺癌细胞系和一些新鲜甲状腺肿瘤中c-myc mRNA水平更高。在最恶性的细胞系和未分化的人甲状腺癌中增加最为明显。用myc特异性反义寡核苷酸阻断c-MYC蛋白合成可显著降低甲状腺癌细胞系的生长速率。我们的结果表明，c-myc过表达在甲状腺癌细胞生长中起关键作用，这支持了myc原癌基因可能参与甲状腺癌发生的肿瘤进展这一假说。
