Avet-Loiseau H, Devilder M C, Garand R, Bouyge I, Rapp M J, Milpied N, Harousseau J L, Moisan J P, Bataille R
Laboratory of Hematology, Laboratory of Molecular Genetics, and Department of Clinical Hematology, University Hospital, 44035 Nantes, Cedex, France.
Clin Cancer Res. 1996 Oct;2(10):1673-7.
Fluorescence in situ hybridization with a chromosome 12-specific alpha-centromeric probe and a 13q14 yeast artificial chromosome probe was performed on interphase cells from 100 patients with B-cell chronic lymphocytic leukemia. Thirty-one patients exhibited a 13q14 deletion. No correlation was found between 13q14 deletions and clinical stage, sex, or morphology. Sixteen patients had trisomy 12, including 6 (of 12) with an atypical morphology. Trisomy 12 and 13q14 abnormalities were detected concomitantly in three patients only. The analysis of patients with deletions clearly showed that in five cases a significant number of cells retained two signals with the yeast artificial chromosome probe, indicating a genetic heterogeneity among the leukemic population. Our data confirm that the 13q14 deletion is a frequent event, indicate that the concomitant occurrence of 13q14 deletion and trisomy 12 is rare but possible, and show that both abnormalities are secondary events in B-cell chronic lymphocytic leukemia.
运用12号染色体特异性α-着丝粒探针和13q14酵母人工染色体探针，对100例B细胞慢性淋巴细胞白血病患者的间期细胞进行荧光原位杂交检测。31例患者出现13q14缺失。未发现13q14缺失与临床分期、性别或形态学之间存在相关性。16例患者存在12号染色体三体，其中12例中有6例具有非典型形态。仅在3例患者中同时检测到12号染色体三体和13q14异常。对缺失患者的分析清楚地表明，在5例患者中，大量细胞保留了酵母人工染色体探针的两个信号，这表明白血病群体中存在遗传异质性。我们的数据证实13q14缺失是常见事件，表明13q14缺失和12号染色体三体同时出现虽罕见但有可能，并且表明这两种异常在B细胞慢性淋巴细胞白血病中均为继发事件。
