Kitagawa Y, Ueda M, Ando N, Ozawa S, Shimizu N, Kitajima M
Departments of Surgery and Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan.
Clin Cancer Res. 1996 May;2(5):909-14.
To determine the value of epidermal growth factor receptor (EGFR) gene amplification as a biological prognostic indicator in patients with esophageal squamous cell carcinoma, the EGFR gene amplification was determined by slot-blot hybridization using DNA extracted from formalin-fixed and paraffin-embedded blocks of tissues from 107 patients with esophageal squamous cell carcinoma who had undergone curative surgery. Results were analyzed by both univariate and multivariate statistical analysis. EGFR gene amplification greater than 3-fold was detected in the primary tumors of 13 (12%) of the 107 cases. The cumulative survival rate for patients with EGFR gene amplification in the primary tumors was significantly lower than that for patients without amplification (P < 0.001). A significant correlation was observed between extensive lymph node involvement at the time of surgery and EGFR gene amplification (P < 0.05). In the multivariate analysis, EGFR gene amplification (P = 0.015) and vascular invasion (P = 0.003) proved to retain independent prognostic values. These results suggest that EGFR gene amplification may be a useful biological marker for the prediction of lymph node metastasis and a poorer prognosis of esophageal squamous cell carcinoma.
为了确定表皮生长因子受体（EGFR）基因扩增作为食管鳞状细胞癌患者生物学预后指标的价值，采用斑点杂交法，对107例接受根治性手术的食管鳞状细胞癌患者经福尔马林固定、石蜡包埋组织块提取的DNA进行检测，以确定EGFR基因扩增情况。采用单因素和多因素统计分析方法对结果进行分析。107例患者中，13例（12%）原发肿瘤检测到EGFR基因扩增超过3倍。原发肿瘤有EGFR基因扩增患者的累积生存率显著低于无扩增患者（P < 0.001）。手术时广泛淋巴结受累与EGFR基因扩增之间存在显著相关性（P < 0.05）。多因素分析显示，EGFR基因扩增（P = 0.015）和血管侵犯（P = 0.003）具有独立的预后价值。这些结果表明，EGFR基因扩增可能是预测食管鳞状细胞癌淋巴结转移及预后较差的有用生物学标志物。
