Fong K M, Kida Y, Zimmerman P V, Smith P J
Queensland Cancer Fund Research Unit, Department of Pathology, University of Queensland Medical School, Herston, Queensland 4006, Australia.
Clin Cancer Res. 1996 Aug;2(8):1369-72.
The tumor inhibitor of metalloproteinase (TIMP) family is a natural inhibitor of several matrix metalloproteinase enzymes which are involved in the process of tumor cell invasion through the extracellular matrix. The aim of this study was to examine TIMP1 RNA expression levels in relation to the clinicopathological features in resected primary non-small cell lung cancer (NSCLC). Total cellular RNA, obtained from 45 cases of NSCLC and adjacent normal lung tissue, was examined using Northern blot analysis. TIMP1 RNA expression levels were heterogeneous in NSCLC but was significantly higher in the adenocarcinoma compared to the squamous cell carcinoma subtype. Although the TIMP1 RNA levels did not correlate with sex, smoking, tumor, node, or TNM stage, there was a statistically significant survival disadvantage for cases with relatively high TIMP1 RNA expression, suggesting a role for TIMP1 in determining the prognosis of resected NSCLC.
金属蛋白酶组织抑制剂（TIMP）家族是几种基质金属蛋白酶的天然抑制剂，这些酶参与肿瘤细胞通过细胞外基质的侵袭过程。本研究的目的是检测TIMP1 RNA表达水平与手术切除的原发性非小细胞肺癌（NSCLC）临床病理特征的关系。使用Northern印迹分析检测从45例NSCLC及相邻正常肺组织中获取的总细胞RNA。TIMP1 RNA表达水平在NSCLC中存在异质性，但腺癌中的表达水平明显高于鳞状细胞癌亚型。虽然TIMP1 RNA水平与性别、吸烟、肿瘤、淋巴结或TNM分期无关，但TIMP1 RNA表达相对较高的病例存在统计学上显著的生存劣势，提示TIMP1在决定手术切除的NSCLC预后中发挥作用。
