Mazanowska O, Klinger M, Wendycz-Domalewska D, Kopeć W
Katedra i Klinika Nefrologii Akademii Medycznej we Wrocławiu.
Pol Arch Med Wewn. 1998 May;99(5):390-7.
We analysed the occurrence of anti-neutrophil cytoplasmic antibodies (ANCA) in sera of 191 patients with glomerulonephritis (76 females and 115 males) by the standard indirect immunofluorescence method (IIF). The presence of ANCA was demonstrated in sera of 4.4% (8/181) patients with idiopathic glomerulonephritis (GN) and in 30% (3/10) of patients with rapidly progressive glomerulonephritis (RPGN), as a form of renal limited vasculitis. In the experimental part of our study we analysed the influence of GN ANCA-negative sera on the neutrophil function in vitro and compared with the effect of ANCA-positive sera (titre > or = 4:40) from systemic vasculitis (SV) patients with renal involvement. The activation of neutrophils was established by reactive oxygen species (ROS) production and the ability of superoxide anion to reduce ferrocytochrome c. Among 30 ANCA-negative GN sera 20% (6/30) revealed the ability to activate neutrophils isolated from healthy donor. Remaining ANCA-negative GN sera and all sera from normal healthy individuals (negative control group) did not affect the neutrophil function and did not induce the superoxide anion production. Their effect was similar to the second negative reference system without serum. Only 33% (3/9) of high titre ANCA-positive sera (> or = 1:40) from SV patients were able to activate neutrophils and to produce the superoxide anion with following ferrocytochrome c reduction, but the effect of activation was most powerfully expressed (three times greater than by GN ANCA-negative sera). The remaining ANCA-positive sera and all SV ANCA-negative sera did not affect the neutrophil function in vitro. These experimental data indicate that the presence of ANCA in GN sera is not necessary to induce neutrophil activation in vitro. On the other hand the influence of the SV ANCA-positive sera was most powerful expressed, although only 33% of sera were able to activate neutrophils in vitro. Our results indicate that not always ANCA presence in serum was connected with the ability to neutrophil activation in vitro. It is possible that in ANCA-negative sera other factors were able to activate neutrophils in vitro, but the effect of activation was markedly lower.
我们采用标准间接免疫荧光法（IIF）分析了191例肾小球肾炎患者（76例女性和115例男性）血清中抗中性粒细胞胞浆抗体（ANCA）的发生情况。在特发性肾小球肾炎（GN）患者的血清中，4.4%（8/181）检测到ANCA，在快速进行性肾小球肾炎（RPGN）患者中，30%（3/10）检测到ANCA，后者为肾局限性血管炎的一种形式。在我们研究的实验部分，我们分析了GN患者ANCA阴性血清对体外中性粒细胞功能的影响，并与有肾脏受累的系统性血管炎（SV）患者的ANCA阳性血清（滴度≥4:40）的作用进行比较。通过活性氧（ROS）生成以及超氧阴离子还原亚铁细胞色素c的能力来确定中性粒细胞的激活情况。在30份ANCA阴性的GN血清中，20%（6/30）显示出激活从健康供体分离出的中性粒细胞的能力。其余ANCA阴性的GN血清以及所有正常健康个体的血清（阴性对照组）均不影响中性粒细胞功能，也不诱导超氧阴离子生成。它们的作用与不含血清的第二个阴性参照体系相似。来自SV患者的高滴度ANCA阳性血清（≥1:40）中，只有33%（3/9）能够激活中性粒细胞并产生超氧阴离子，随后使亚铁细胞色素c还原，但其激活作用最为显著（比GN患者ANCA阴性血清强三倍）。其余ANCA阳性血清以及所有SV患者ANCA阴性血清在体外均不影响中性粒细胞功能。这些实验数据表明，GN血清中ANCA的存在并非体外诱导中性粒细胞激活所必需。另一方面，SV患者ANCA阳性血清的影响最为显著，尽管只有33%的血清能够在体外激活中性粒细胞。我们的结果表明，血清中ANCA的存在并不总是与体外中性粒细胞激活能力相关。有可能在ANCA阴性血清中，其他因素能够在体外激活中性粒细胞，但激活作用明显较低。
