Yang J P, Finkelman M A, Clarke M W
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
J Urol. 1998 Dec;160(6 Pt 1):2240-4. doi: 10.1097/00005392-199812010-00093.
To evaluate the expression of prostate secretory protein of 94 amino acids (PSP94) and PSP94 binding proteins in the LNCaP cell line.
The reverse-transcription polymerase chain reaction (RT-PCR) and Southern blot hybridization were employed to assay the expression of PSP94. Immunoprecipitation with specific polyclonal antibodies was used to detect PSP94 secreted by the LNCaP cells. The binding proteins were assayed by equilibrium binding assays.
PSP94 was expressed and secreted in the LNCaP cells. As well as, LNCaP cells expressed surface membrane proteins capable of binding PSP94 in a specific and saturable manner. Exposure of LNCaP cells to exogenous PSP94 resulted in the up-regulation of PSP94 binding sites, indicating functional interactions for PSP94 and its receptor in this cell line.
The expression of PSP94 and its receptors may be partially regulated by an autocrine pathway in the LNCaP cell line.
评估94个氨基酸的前列腺分泌蛋白（PSP94）及其结合蛋白在LNCaP细胞系中的表达情况。
采用逆转录聚合酶链反应（RT-PCR）和Southern印迹杂交法检测PSP94的表达。用特异性多克隆抗体进行免疫沉淀以检测LNCaP细胞分泌的PSP94。通过平衡结合试验检测结合蛋白。
PSP94在LNCaP细胞中表达并分泌。此外，LNCaP细胞表达能够以特异性和饱和方式结合PSP94的表面膜蛋白。将LNCaP细胞暴露于外源性PSP94会导致PSP94结合位点上调，表明在该细胞系中PSP94与其受体存在功能相互作用。
在LNCaP细胞系中，PSP94及其受体的表达可能部分受自分泌途径调控。
