Montuschi P, Ciabattoni G, Paredi P, Pantelidis P, du Bois R M, Kharitonov S A, Barnes P J
Imperial College School of Medicine at the National Heart and Lung Institute, Department of Thoracic Medicine, Interstitial Lung Disease Unit, Royal Brompton Hospital, London, United Kingdom.
Am J Respir Crit Care Med. 1998 Nov;158(5 Pt 1):1524-7. doi: 10.1164/ajrccm.158.5.9803102.
Oxidative stress contributes to the pathophysiology of interstitial lung diseases, such as cryptogenic fibrosing alveolitis (CFA), fibrosing alveolitis associated with systemic sclerosis (FASSc) and sarcoidosis. F2-isoprostanes are a series of prostaglandin (PG) F2-like compounds produced in vivo independent of cyclooxygenase, as products of the radical-catalyzed lipid peroxidation. Measurement of the concentrations of F2-isoprostanes has proved to be valuable in assessing oxidative stress in vivo. The aim of this study was to measure 8-epi-PGF2alpha concentrations, one of the most abundant F2-isoprostane in humans, in bronchoalveolar lavage (BAL) in normal subjects and to compare them to those observed in patients with CFA (n = 9), FASSc (n = 8) and sarcoidosis (n = 10). 8-epi-PGF2alpha was detectable in BAL fluid in normal subjects (9.6 +/- 0.8 pg/ml) and its concentrations were increased approximately 5-fold in patients with CFA (47.4 +/- 7.0, p < 0.001) and FASSc (43.2 +/- 3.3, p < 0. 001). 8-epi-PGF2alpha was also increased in patients with sarcoidosis, although to a lesser extent (12.0 +/- 0.70 pg/ml, p < 0. 01). No correlation between 8-epi-PGF2alpha and either lung function tests or BAL cell types was observed in any group of patients. Our study shows that the level of oxidative stress is enhanced in patients with interstitial lung diseases as reflected by increased concentrations of 8-epi-PGF2alpha in BAL fluid.
氧化应激参与了间质性肺疾病的病理生理过程，如隐源性纤维性肺泡炎（CFA）、与系统性硬化症相关的纤维性肺泡炎（FASSc）和结节病。F2-异前列腺素是体内一系列独立于环氧化酶产生的类前列腺素（PG）F2化合物，是自由基催化脂质过氧化的产物。事实证明，测量F2-异前列腺素的浓度对于评估体内氧化应激具有重要价值。本研究的目的是测量正常受试者支气管肺泡灌洗（BAL）中8-表-前列腺素F2α（8-epi-PGF2α）的浓度，8-epi-PGF2α是人体内含量最丰富的F2-异前列腺素之一，并将其与CFA患者（n = 9）、FASSc患者（n = 8）和结节病患者（n = 10）的测量结果进行比较。正常受试者的BAL液中可检测到8-epi-PGF2α（9.6±0.8 pg/ml），CFA患者（47.4±7.0，p < 0.001）和FASSc患者（43.2±3.3，p < 0.001）的浓度增加了约5倍。结节病患者的8-epi-PGF2α也有所增加，尽管增幅较小（12.0±0.70 pg/ml，p < 0.01）。在任何一组患者中，均未观察到8-epi-PGF2α与肺功能测试或BAL细胞类型之间存在相关性。我们的研究表明，间质性肺疾病患者的氧化应激水平升高，这在BAL液中8-epi-PGF2α浓度升高上得到了体现。
