Heller H, Schuster K
Department of Physiology, University of Bonn, Nussallee 11, D-53115 Bonn, Germany.
Pflugers Arch. 1998 Dec;437(1):94-7. doi: 10.1007/s004240050752.
To evaluate whether nitric oxide (NO) is an appropriate test gas for assessing pulmonary gas exchange, we determined the rates of disappearance from the alveolar space (lambda) of NO and singly and doubly 18O-labelled carbon dioxide (C16O18O, C18O2) by performing single-breath manoeuvres on seven artificially ventilated rabbits. By exploiting unique features of both isotopic species and by analysing pulmonary gas transport and lambda values with a commonly used model, we found that diffusion forms 98+/-6% (mean +/- SD) of the overall resistance to alveolar-capillary NO transfer. This means that measurements of pulmonary NO uptake reveal the entire diffusive properties of the alveolar-capillary membrane, because the extremely fast binding of NO to haemoglobin negates the "reactive" component within red blood cells of pulmonary capillaries.
为了评估一氧化氮（NO）是否是用于评估肺气体交换的合适测试气体，我们对七只人工通气的兔子进行了单次呼吸动作，测定了NO以及单标记和双标记18O的二氧化碳（C16O18O、C18O2）从肺泡腔消失的速率（λ）。通过利用这两种同位素的独特特性，并使用常用模型分析肺气体传输和λ值，我们发现扩散占肺泡-毛细血管NO转移总阻力的98±6%（平均值±标准差）。这意味着肺NO摄取的测量揭示了肺泡-毛细血管膜的全部扩散特性，因为NO与血红蛋白的结合极快，消除了肺毛细血管红细胞内的“反应性”成分。
