通过平衡超速离心法检测核酸-蛋白质复合物
Detection of nucleic acid-protein complexes by equilibrium ultracentrifugation.
作者信息
Lanks K W, Eng R K
出版信息
Res Commun Chem Pathol Pharmacol. 1976 Oct;15(2):377-80.
PMID:981795
Abstract
Formation of complexes between yeast phenylalanine transfer RNA and phenylalanyl aminoacyl-transfer RNA synthetase is shown to occur under equilibrium conditions in the analytical ultracentrifuge. The technique of equilibrium sedimentation should be useful for the detection of transient associations between proteins and nucleic acids when only small amounts of unlabeled, but highly purified, materials are available.
摘要
酵母苯丙氨酸转移核糖核酸与苯丙氨酰氨酰转移核糖核酸合成酶之间复合物的形成，在分析超速离心机的平衡条件下被证明会发生。当只有少量未标记但高度纯化的材料可用时，平衡沉降技术对于检测蛋白质与核酸之间的瞬时结合应该是有用的。
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Detection of nucleic acid-protein complexes by equilibrium ultracentrifugation.通过平衡超速离心法检测核酸-蛋白质复合物
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