Falkenberg M, Björnheden T, Odén A, Risberg B
Department of Vascular Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden.
Eur J Vasc Endovasc Surg. 1998 Oct;16(4):276-83. doi: 10.1016/s1078-5884(98)80046-4.
To assess the representativity of small vascular samples with regard to the expression of six important factors in atherosclerotic vessels.
Circumferential frozen sections of iliac and femoral arteries (n = 9) from patients undergoing reconstructive bypass surgery.
Immunohistochemistry with antibodies against macrophages (CD68), tumour necrosis factor alpha, tissue factor, tissue plasminogen activator, urokinase plasminogen activator and plasminogen activator inhibitor type 1. The distribution of these antigens was characterised by computer assisted image analysis. Antigen positive area in randomly chosen samples of varying size was compared with antigen positive area in the whole vessel transections.
Marked heterogeneity was found with respect to the expression of these factors in atherosclerotic vessels. The representativity of samples was highly dependent on the size of the samples. PAI-1 was more evenly expressed compared to the other antigens.
Estimates of the expression of these factors based on small samples from atherosclerotic arteries are unreliable.
评估小血管样本在动脉粥样硬化血管中六个重要因子表达方面的代表性。
来自接受重建性搭桥手术患者的髂动脉和股动脉的环形冰冻切片（n = 9）。
使用针对巨噬细胞（CD68）、肿瘤坏死因子α、组织因子、组织型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂和纤溶酶原激活剂抑制剂1型的抗体进行免疫组织化学。通过计算机辅助图像分析对这些抗原的分布进行表征。将不同大小的随机选择样本中的抗原阳性面积与整个血管横切面中的抗原阳性面积进行比较。
在动脉粥样硬化血管中，这些因子的表达存在明显的异质性。样本的代表性高度依赖于样本大小。与其他抗原相比，PAI-1的表达更为均匀。
基于动脉粥样硬化动脉小样本对这些因子表达的估计是不可靠的。
