Kaji Y, Obata H, Usui T, Soya K, Machinami R, Tsuru T, Yamashita H
Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
J Cataract Refract Surg. 1998 Nov;24(11):1441-6. doi: 10.1016/s0886-3350(98)80164-8.
To investigate the structural changes in corneal stromal collagen fibrils after excimer laser keratectomy in relation to the degree of corneal haze.
University of Tokyo Hospital, Tokyo, Japan.
Corneal haze was quantitatively measured by analyzing the light scattering in Scheimpflug images of the corneas of white rabbits after excimer laser keratectomy. Collagen fibril structure was examined using scanning electron microscopy after chemical digestion with sodium hydroxide solution; the same specimens were examined by transmission electron microscopy after re-embedding.
Corneal haze reached a peak 4 weeks after excimer laser keratectomy and then gradually decreased. The collagen fibrils of the normal cornea were regularly arranged parallel to the surface of the cornea, with small interfibrillar distances. After excimer laser keratectomy, the arrangement was highly disordered, with increased interfibrillar distances. These structural changes were most prominent 4 weeks after excimer laser keratectomy.
The structural changes in the collagen fibrils of the corneal stroma, especially the increase in interfibrillar distances and the disordered arrangement, were associated with corneal haze after excimer laser keratectomy.
研究准分子激光角膜切削术后角膜基质胶原纤维的结构变化及其与角膜混浊程度的关系。
日本东京东京大学医院。
通过分析准分子激光角膜切削术后白兔角膜的Scheimpflug图像中的光散射来定量测量角膜混浊。用氢氧化钠溶液化学消化后，使用扫描电子显微镜检查胶原纤维结构；重新包埋后，对相同标本进行透射电子显微镜检查。
准分子激光角膜切削术后4周角膜混浊达到峰值，然后逐渐下降。正常角膜的胶原纤维与角膜表面平行排列规则，纤维间距离小。准分子激光角膜切削术后，排列高度紊乱，纤维间距离增加。这些结构变化在准分子激光角膜切削术后4周最为明显。
角膜基质胶原纤维的结构变化，尤其是纤维间距离增加和排列紊乱，与准分子激光角膜切削术后的角膜混浊有关。
