Muramatsu N, Nakauchi K
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
J Microencapsul. 1998 Nov-Dec;15(6):715-23. doi: 10.3109/02652049809008254.
The membrane emulsification method was adopted to prepare monodisperse albumin microspheres. Uniform-sized emulsion droplets were successfully obtained by passing albumin solution through the membrane into kerosene at appropriate applied pressures. After heating the emulsion, monodisperse albumin microspheres were prepared and the heat-denaturing process itself did not affect their monodispersity once uniform emulsion droplets had been prepared. The shape and size of the albumin microspheres were strongly dependent on the concentration of the albumin solution and the heat denaturing temperature. Lower albumin concentrations produced non-spherical particles and higher denaturing temperatures made the particles smaller. Incorporated riboflavin released quite rapidly from the albumin microspheres, and increases in both albumin concentration and denaturing temperature caused the drug release to be sustained, presumably because a rigid and dense matrix was formed within the albumin microspheres.
采用膜乳化法制备单分散白蛋白微球。通过在适当的外加压力下使白蛋白溶液透过膜进入煤油中，成功获得了尺寸均匀的乳液滴。加热乳液后，制备出了单分散白蛋白微球，并且一旦制备出均匀的乳液滴，热变性过程本身并不影响其单分散性。白蛋白微球的形状和大小强烈依赖于白蛋白溶液的浓度和热变性温度。较低的白蛋白浓度会产生非球形颗粒，而较高的变性温度会使颗粒变小。包封的核黄素从白蛋白微球中释放得相当快，白蛋白浓度和变性温度的增加都会导致药物释放持续，推测这是因为在白蛋白微球内形成了刚性且致密的基质。
