通过ELISA法检测小鼠毛发中乙醛加合物来测定乙醇摄入量。

Detection of ethanol consumption by ELISA assay measurement of acetaldehyde adducts in murine hair.

作者信息

Watson R R, Solkoff D, Wang J Y, Seeto K

机构信息

Arizona Prevention Center, University of Arizona, Tucson 85724, USA.

出版信息

Alcohol. 1998 Nov;16(4):279-84. doi: 10.1016/s0741-8329(98)00014-7.

DOI:10.1016/s0741-8329(98)00014-7

PMID:9818979

Abstract

Acetaldehyde (AA), a principal metabolite of ethanol, reacts with proteins to form protein-AA adducts in vivo and in vitro. We studied three different protein-AA adducts produced in vitro, as well as hair keratin-AA adducts from ethanol-fed and/or cocaine-injected mice. An enzyme linked immunosorbent assay (ELISA) was developed that detected the stable protein-AA adducts in protein isolated from hair. Cocaine injection had little effect on hair AA adduct formation. The indirect ELISA assay showed significantly increased levels of keratin-AA adducts in hair from mice fed ethanol for 8 weeks. Hair collection was noninvasive and the AA-protein adducts were stable. Therefore, this indirect ELISA assay could be further developed as a biochemical parameter for alcoholism in the clinical setting.

摘要

乙醛（AA）是乙醇的主要代谢产物，在体内和体外均可与蛋白质反应形成蛋白质-AA加合物。我们研究了三种体外产生的不同蛋白质-AA加合物，以及来自喂食乙醇和/或注射可卡因小鼠的毛发角蛋白-AA加合物。开发了一种酶联免疫吸附测定（ELISA）方法，用于检测从毛发中分离出的蛋白质中的稳定蛋白质-AA加合物。注射可卡因对毛发AA加合物的形成影响不大。间接ELISA分析显示，喂食乙醇8周的小鼠毛发中的角蛋白-AA加合物水平显著升高。毛发采集是非侵入性的，且AA-蛋白质加合物稳定。因此，这种间接ELISA分析可进一步开发为临床环境中酒精中毒的生化参数。