Tucková E, Svobodová J, Anisimová E, Doleźalová B, Vonka V, Stárek M
Acta Virol. 1976 Aug;20(4):305-12.
After passaging the NWS influenza virus at increasing temperatures in the hamster embryo fibroblast (HEF) cell line, a temperature-resistant (tr) mutant which grew well at 39 degrees C was isolated. Attempts were made to isolate temperature-sensitive (ts) mutants from the tr virus. After growth in the presence of 5-fluorouracil, 233 virus clones were isolated. Among these only one was incapable of replicating at 39 degrees C. The physiological studies, the shift-up experiment and the electron microscopic investigation suggested that the genetic defect responsible for its ts character influenced a late function of the virus genome. In the lungs of intranasally infected mice, the ts mutant replicated to a lesser extent than the tr virus. During its growth in vivo, the ts virus exhibited a high degree of genetic stability.
在仓鼠胚胎成纤维细胞（HEF）系中于不断升高的温度下传代NWS流感病毒后，分离出了一种在39℃能良好生长的耐温（tr）突变体。尝试从tr病毒中分离温度敏感（ts）突变体。在5-氟尿嘧啶存在的情况下培养后，分离出233个病毒克隆。其中只有一个在39℃不能复制。生理学研究、温度上调实验和电子显微镜研究表明，导致其ts特性的基因缺陷影响了病毒基因组的晚期功能。在经鼻感染小鼠的肺中，ts突变体的复制程度低于tr病毒。在其体内生长过程中，ts病毒表现出高度的遗传稳定性。
