Bell I, Ashman C, Maughan J, Hooker E, Cook F, Reinhart T A
Immunopathology Unit, Glaxo Wellcome Medicines Research Centre, Stevenage, UK.
J Gen Virol. 1998 Nov;79 ( Pt 11):2717-27. doi: 10.1099/0022-1317-79-11-2717.
The Nef protein of simian immunodeficiency virus (SIV) is dispensable for replication in established T-cell lines but essential for high level virus replication in the adult host, though the mechanism by which Nef contributes to this has remained unclear. We demonstrate here that SIV Nef binds to the zeta chain of the T-cell receptor (TCR). SIV Nef proteins that interact with TCR zeta in a yeast two-hybrid system also reduce T-cell surface expression levels of TCR alphabeta, CD3 and CD4. These findings are the first demonstration that Nef can bind directly to a component of the TCR-CD3 complex and modulate its surface expression.
猿猴免疫缺陷病毒（SIV）的Nef蛋白对于在已建立的T细胞系中复制而言并非必需，但对于成年宿主中的高水平病毒复制却是必不可少的，尽管Nef促成此过程的机制仍不清楚。我们在此证明，SIV Nef与T细胞受体（TCR）的ζ链结合。在酵母双杂交系统中与TCR ζ相互作用的SIV Nef蛋白也会降低TCR αβ、CD3和CD4的T细胞表面表达水平。这些发现首次证明Nef可直接结合至TCR-CD3复合物的一个组分并调节其表面表达。
