Kobayashi T, Kajiura-Kobayashi H, Nagahama Y
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Japan.
Mol Reprod Dev. 1998 Dec;51(4):355-61. doi: 10.1002/(SICI)1098-2795(199812)51:4<355::AID-MRD1>3.0.CO;2-G.
We produced an antibody that recognized only early stages of spermatogonia in Japanese eel testis. This antibody (anti-spermatogonia-specific antigen-1, anti-SGSA-1) recognized a band of about 38 kDa in Western blot analysis of extracts from eel testis. This antigen was observed by immunohistochemistry only in type-A and early type-B spermatogonia and could not be seen in the late type-B spermatogonia, which appeared after the initiation of spermatogenesis by a single injection of human chorionic gonadotropin. Immunoreactive SGSA-1 was absent in spermatocytes, spermatids, spermatozoa, Sertoli cells, and interstitial Leydig cells. Similarly, this antigen was also detected only in type-A/primary spermatogonia in the testes of two species of teleosts, medaka (Oryzias latipes) and tilapia (Oreochromis niloticus), as well as a toad (Xenopus laevis). These results imply that the disappearance of SGSA-1 in late type-B/secondary spermatogonia is a critical step in the progression of spermatogenesis, and indicate that anti-SGSA-1 is a useful marker for analysis of the molecular mechanism controlling the differentiation of spermatogonia in lower vertebrates.
我们制备了一种仅识别日本鳗鲡睾丸中精原细胞早期阶段的抗体。在对鳗鲡睾丸提取物进行的蛋白质印迹分析中，这种抗体（抗精原细胞特异性抗原-1，抗-SGSA-1）识别出一条约38 kDa的条带。通过免疫组织化学观察到，这种抗原仅存在于A型和早期B型精原细胞中，而在单次注射人绒毛膜促性腺激素启动精子发生后出现的晚期B型精原细胞中未见。免疫反应性SGSA-1在精母细胞、精子细胞、精子、支持细胞和间质Leydig细胞中均不存在。同样，在两种硬骨鱼（青鳉（Oryzias latipes）和罗非鱼（Oreochromis niloticus））以及蟾蜍（非洲爪蟾（Xenopus laevis））的睾丸中，也仅在A型/初级精原细胞中检测到这种抗原。这些结果表明，SGSA-1在晚期B型/次级精原细胞中的消失是精子发生进程中的关键步骤，并表明抗-SGSA-1是分析低等脊椎动物精原细胞分化分子机制的有用标志物。
