Tosswill J H, Taylor G P, Clewley J P, Weber J N
Hepatitis and Retrovirus Laboratory, Central Public Health Laboratory, London, UK.
J Virol Methods. 1998 Nov;75(1):21-6. doi: 10.1016/s0166-0934(98)00093-7.
A nested PCR was designed using primers from the pol and tax genes of human T-cell leukaemia virus type I (HTLV-I). The assay reliably detected a single copy of HTLV-I proviral genome in DNA from 1 x 10(5) Peripheral blood mononuclear cells (PBMCs). Using serial dilutions of sample DNA, the assay was applied prospectively to study proviral load in patients with HTLV-associated disease and carriers. The median proviral load expressed as number of copies/100 PBMCs was found to be 14.0 copies in patients with HAM and 1.55 copies in initially asymptomatic carriers. The assay was used to test for low proviral load in subjects who may have HTLV-I infection, and to monitor response to therapy.
设计了一种巢式聚合酶链反应（PCR），使用来自I型人类T细胞白血病病毒（HTLV-I）的pol和tax基因的引物。该检测方法能够可靠地检测出1×10⁵外周血单个核细胞（PBMC）DNA中HTLV-I前病毒基因组的单拷贝。通过对样本DNA进行系列稀释，该检测方法被前瞻性地应用于研究HTLV相关疾病患者和携带者的前病毒载量。发现HAM患者中以前病毒拷贝数/100个PBMC表示的前病毒载量中位数为14.0拷贝，初始无症状携带者为1.55拷贝。该检测方法用于检测可能感染HTLV-I的受试者的低前病毒载量，并监测治疗反应。
