Ischemic preconditioning versus intermittent reperfusion to improve blood flow to a vascular isolated skeletal muscle flap of rats.

作者信息

Wang W Z, Anderson G, Firrell J C, Tsai T M

机构信息

Center for Applied Microcirculatory Research, University of Louisville, and Christine M. Kleinert Institute for Hand and Micro Surgery, Kentucky 40292, USA.

出版信息

J Trauma. 1998 Nov;45(5):953-9. doi: 10.1097/00005373-199811000-00018.

DOI:10.1097/00005373-199811000-00018

PMID:9820708

Abstract

OBJECTIVES

We investigated whether intermittent restoration of blood flow just before reperfusion after ischemia could create beneficial effects similar to ischemic preconditioning, which involves intermittent stoppage of blood flow just before ischemia.

METHODS

Male Sprague-Dawley rats were prepared with vascular isolated cremaster muscles, then subjected to 4 hours of ischemia and 60 minutes of reperfusion. Arteriole diameters and capillary perfusion were measured by using intravital microscopy. Four groups were used: (1) untreated, (2) ischemic preconditioning (IP), (3) intermittent reperfusion (IR), and (4) ischemic preconditioning plus intermittent reperfusion (IP+IR).

RESULTS

Our results showed that IP significantly attenuated both ischemia/reperfusion-induced vasospasm and capillary noreflow. IR was effective in attenuating vasospasm in terminal arterioles. However, IR alone was unable to significantly attenuate capillary no-reflow. Combining both IP and IR achieved the best results.