腐殖酸在毛细管内壁的毛细管区带电泳吸附、在添加金属离子的缓冲液中的分离及指纹图谱

Humic acid capillary zone electrophoresis adsorption on capillary walls, separation in metal ion supplemented buffer and the fingerprints.

作者信息

Fetsch D, Fetsch M, Peña Mendéz E M, Havel J

机构信息

Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.

出版信息

Electrophoresis. 1998 Oct;19(14):2465-73. doi: 10.1002/elps.1150191419.

DOI:10.1002/elps.1150191419

PMID:9820969

Abstract

Capillary zone electrophoresis (CZE) in quartz tubes is often being used for the separation and characterization of humic acids (HA). A method was found to follow adsorption (and kinetics) of humic acids on a fused-silica capillary wall. It was shown that the adsorption of humic acids on an uncoated capillary wall is high. The effect on sorption of additives to the background electrolyte (BGE) was studied. Sorption can be eliminated by adding magnesium(II) salts (14-50 mM) to the BGE (pH 3.40) with resultant highly reproducible electropherograms as well as detailed and expressive fingerprints for HA of different origin.

摘要

石英管中的毛细管区带电泳（CZE）常被用于腐殖酸（HA）的分离和表征。已发现一种方法可追踪腐殖酸在熔融石英毛细管管壁上的吸附（及动力学）。结果表明，腐殖酸在未涂层毛细管管壁上的吸附量很高。研究了背景电解质（BGE）中添加剂对吸附的影响。通过向BGE（pH 3.40）中添加镁（II）盐（14 - 50 mM）可消除吸附，从而得到高度可重现的电泳图以及不同来源HA的详细且有表现力的指纹图谱。