卡诺固定剂可减少脐血来源的人培养肥大细胞免疫细胞化学染色中糜酶阳性细胞的数量。

Carnoy's fixative reduces the number of chymase-positive cells in immunocytochemical staining of cord-blood-derived human cultured mast cells.

作者信息

Kanbe N, Kurosawa M, Miyachi Y, Kanbe M, Kempuraj D, Tachimoto H, Saito H

机构信息

Department of Dermatology, Gunma University School of Medicine, Maebashi, Japan.

出版信息

Allergy. 1998 Oct;53(10):981-5. doi: 10.1111/j.1398-9995.1998.tb03800.x.

DOI:10.1111/j.1398-9995.1998.tb03800.x

PMID:9821479

Abstract

KleinJan et al. (Allergy 1996;51:614-20) reported that Carnoy's fixative reduced the number of chymase-positive mast cells in the nasal mucosa. Therefore, in the present study, we investigated whether Carnoy's fixative reduces the number of chymase-positive cells from cord-blood-derived human cultured mast cells when compared with other types of fixatives. Human mast cells were obtained by culturing cord-blood-derived CD34-positive cells in the presence of stem cell factor and interleukin-6. Staining procedures of the cells in fixation with Carnoy's fixative and with other fixatives gave no differences among the number of tryptase-positive cells, whereas fixation with Carnoy's fixative for 15 min gave a significant decrease in the number of chymase-positive cells compared with acetone for 10 min. The number of chymase-positive cells decreased in a time-dependent manner under fixation with Carnoy's fixative, indicating that Carnoy's fixative had a negative effect on the number of chymase-positive cells from cord-blood-derived human cultured mast cells.

摘要

克莱因扬等人（《过敏》，1996年；51卷：614 - 620页）报告称，卡诺氏固定液可减少鼻黏膜中糜酶阳性肥大细胞的数量。因此，在本研究中，我们调查了与其他类型的固定液相比，卡诺氏固定液是否会减少源自脐带血的人培养肥大细胞中糜酶阳性细胞的数量。通过在干细胞因子和白细胞介素 - 6存在的情况下培养源自脐带血的CD34阳性细胞来获得人肥大细胞。用卡诺氏固定液和其他固定液固定细胞的染色程序在类胰蛋白酶阳性细胞数量上没有差异，而与用丙酮固定10分钟相比，用卡诺氏固定液固定15分钟时糜酶阳性细胞数量显著减少。在用卡诺氏固定液固定的情况下，糜酶阳性细胞数量呈时间依赖性减少，这表明卡诺氏固定液对源自脐带血的人培养肥大细胞中糜酶阳性细胞的数量有负面影响。