Lee S Y, Kim D S, Kim K E, Jeaung B J, Lee K Y
Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Med J. 1998 Oct;39(5):409-16. doi: 10.3349/ymj.1998.39.5.409.
It is widely known that the cockroach is an inhalant allergen in atopic asthma and allergic rhinitis. Even though Bla g I and Bla g II are considered as the major allergens, several relatively high-molecular weight (MW) cockroach allergens have also been recently identified by IgE-immunoblot in western countries. However, the environmental control and diagnostic tests mainly focussed on Bla g I and Bla g II. Furthermore there is no data about major IgE-binding cockroach antigens in Korea. We performed this study to identify the major German cockroach allergens in Korean atopic children. By the results of allergy skin tests, 14 children with atopic asthma (9 were cockroach-sensitive and 5 were cockroach-nonsensitive atopics) were enrolled in this study. We conducted IgE immunoblot and autoradiographic analysis using Yonsei-extract of German cockroach antigen produced in our laboratory, individual sera from 9 cockroach-sensitive children, and the pooled sera of 5 house-dust-mites-only-sensitive children. We performed an allergic skin test to cockroach mix, and a radioallergosorbent test (RAST) using German cockroach crude extract on all subjects. German cockroach-specific IgE was detected in 6 out of 9 subjects by RAST. We identified at least 15 IgE-binding protein bands, and among them, the components of MWs of 76, 64, 50, 38, and < 14 kilodaltons (kDa) were the major German cockroach allergens in study subjects. Therefore, Bla g I (25-30 kDa) and Bla g II (36 kDa) could not be the absolute indicators of German cockroach sensitization and parameters of environmental control.
众所周知，蟑螂是特应性哮喘和过敏性鼻炎的吸入性过敏原。尽管Bla g I和Bla g II被认为是主要过敏原，但最近在西方国家通过IgE免疫印迹法也鉴定出了几种相对高分子量（MW）的蟑螂过敏原。然而，环境控制和诊断测试主要集中在Bla g I和Bla g II上。此外，韩国没有关于主要IgE结合蟑螂抗原的数据。我们进行这项研究以确定韩国特应性儿童中主要的德国蟑螂过敏原。根据过敏皮肤试验结果，本研究纳入了14名患有特应性哮喘的儿童（9名对蟑螂敏感，5名对蟑螂不敏感的特应性患者）。我们使用在我们实验室生产的德国蟑螂抗原的延世提取物、9名对蟑螂敏感儿童的个体血清以及5名仅对屋尘螨敏感儿童的混合血清进行了IgE免疫印迹和放射自显影分析。我们对所有受试者进行了蟑螂混合物的过敏性皮肤试验以及使用德国蟑螂粗提物的放射变应原吸附试验（RAST）。通过RAST在9名受试者中的6名中检测到了德国蟑螂特异性IgE。我们鉴定出至少15条IgE结合蛋白带，其中分子量为76、64、50、38和<14千道尔顿（kDa）的成分是研究对象中主要的德国蟑螂过敏原。因此，Bla g I（25 - 30 kDa）和Bla g II（36 kDa）不能成为德国蟑螂致敏和环境控制参数的绝对指标。
