Escribano L, Orfao A, Díaz Agustín B, Cerveró C, Herrero S, Villarrubia J, Bravo P, Torrelo A, Montero T, Valdemoro M, Velasco J L, Navarro J L, San Miguel J F
Department of Hematología, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, Spain.
Cytometry. 1998 Oct 15;34(5):223-8. doi: 10.1002/(sici)1097-0320(19981015)34:5<223::aid-cyto3>3.0.co;2-b.
The quantitative measurement of the expression of both cytoplasmic and surface CD63 antigen by human mast cells from both normal and pathological bone marrow samples was studied by use of flow cytometry. Our major goal was to analyze whether in vivo CD63 expression by human bone marrow mast cells could be useful to discriminate bone marrow mast cells from patients with mastocytosis from other conditions. For that purpose, a total of 65 subjects corresponding to 12 healthy volunteers, 25 B-cell chronic lymphoproliferative disorders, 5 reactive bone marrow samples, 4 myelodysplastic syndromes, and 19 mastocytosis were analyzed. The expression of both surface and cytoplasmic CD63 by human bone marrow mast cells is clearly demonstrated. Our results show that high amounts of CD63 are present in human bone marrow mast cells most of it corresponding to an intracellular localization. No significant differences in CD63 expression were observed as regards both total and cytoplasmic CD63, except for higher CD63 levels in adult patients with mastocytosis (P = 0.05). By contrast, the mean level of surface CD63 significantly varied between the different groups of individuals. Accordingly, patients with monoclonal gammopathies displayed a slight decrease (P = 0.1) in surface CD63 expression, whereas bone marrow mast cells from adults with indolent systemic mast cell disease showed significantly (P = 0.0005) higher levels of surface CD63 as compared to healthy controls.
利用流式细胞术研究了正常和病理骨髓样本中人类肥大细胞胞质和表面CD63抗原表达的定量测定。我们的主要目标是分析人类骨髓肥大细胞在体内的CD63表达是否有助于区分肥大细胞增多症患者的骨髓肥大细胞与其他情况。为此，共分析了65名受试者，包括12名健康志愿者、25例B细胞慢性淋巴细胞增殖性疾病、5例反应性骨髓样本、4例骨髓增生异常综合征和19例肥大细胞增多症。人类骨髓肥大细胞表面和胞质CD63的表达均得到明确证实。我们的结果表明，人类骨髓肥大细胞中存在大量CD63，其中大部分位于细胞内。除成年肥大细胞增多症患者的CD63水平较高外（P = 0.05），总CD63和胞质CD63的表达未观察到显著差异。相比之下，不同个体组之间表面CD63的平均水平有显著差异。因此，单克隆丙种球蛋白病患者的表面CD63表达略有下降（P = 0.1），而与健康对照相比，惰性系统性肥大细胞病成年患者的骨髓肥大细胞表面CD63水平显著升高（P = 0.0005）。
