Hiyama S, Abe K, Mikuni H, Fukuda S, Inuyama Y
Department of Otolaryngology, Hokkaido University School of Medicine, Sapporo, Japan.
Hear Res. 1998 Oct;124(1-2):118-23. doi: 10.1016/s0378-5955(98)00123-3.
This study demonstrated that type A fibrils of the mouse tectorial membrane showed a morphology characteristic of collagen, as demonstrated using an atomic force microscope. In the topographical imaging mode, the surface of the type A fibrils showed a periodic pattern, consisting of alternating grooves and ridges. The periodicity of the type A fibrils was 69.1 +/- 0.6 nm, which is in accordance with characteristic collagen D-periodicity. The difference in height between grooves and ridges was 1.6 +/- 0.3 nm. In the variable deflection imaging mode, the type A fibrils showed a clear banding pattern, which consisted of alternating light and dark zones, with D-periodicity. In addition, the type A fibrils exhibited one minor dark band in the light zone and one minor light band in the dark zone.
本研究表明，使用原子力显微镜观察发现，小鼠盖膜的A型纤维呈现出胶原蛋白特有的形态。在形貌成像模式下，A型纤维的表面呈现出周期性图案，由交替的凹槽和脊组成。A型纤维的周期为69.1±0.6纳米，这与胶原蛋白特有的D周期一致。凹槽和脊之间的高度差为1.6±0.3纳米。在可变偏转成像模式下，A型纤维呈现出清晰的带状图案，由交替的亮区和暗区组成，具有D周期。此外，A型纤维在亮区有一条较窄的暗带，在暗区有一条较窄的亮带。
