Deng Z, Coudray C, Gouzoux L, Mazur A, Rayssiguier Y, Pépin D
Centre de Recherche en Nutrition Humaine d'Auvergne, Centre d'INRA Clermont-Ferrand/Theix, Saint Genès Champanelle, France.
Biol Trace Elem Res. 1998 Aug;63(2):139-47. doi: 10.1007/BF02778873.
Aluminum (Al) absorption seems to be very low, but many factors can enhance it in animals and humans. In the present study, we investigated the acute effect of Na citrate on Al absorption by monitoring Al levels in blood and several tissues. For this purpose, 18 Wistar male rats were divided into 3 groups: control, Al, and Al + Na citrate. After a 14-h fasting period, animals were dosed orally with deionized water, or 2 mmol Al chloride, or 2 mmol Al chloride plus 2 mmol Na citrate. Blood samples were taken before and 1, 2, 4, and 6 h after the gavage. Al concentrations in blood, liver, tibia, kidney, and intestinal wall were determined by ICP-OES. In the Al and Al + citrate groups, Al blood concentrations peaked at 1 h and 2 h with higher levels in the Al + citrate group. Al gavage resulted in an increase in Al level in intestinal wall, but not in the other investigated tissues. Simultaneous gavage of citrate with Al significantly increased its tissue levels in tibia, kidney, and in intestinal wall. Our data show clearly that Al as chloride can be absorbed, but not well retained by the organism tissues. Furthermore, the model used in the present study is appropriate for acute studies to investigate the effect of various compounds on Al absorption in the rat.
铝（Al）的吸收似乎非常低，但许多因素可在动物和人类中增强其吸收。在本研究中，我们通过监测血液和几种组织中的铝水平，研究了柠檬酸钠对铝吸收的急性影响。为此，将18只Wistar雄性大鼠分为3组：对照组、铝组和铝+柠檬酸钠组。在禁食14小时后，给动物口服去离子水、或2 mmol氯化铝、或2 mmol氯化铝加2 mmol柠檬酸钠。在灌胃前以及灌胃后1、2、4和6小时采集血样。通过电感耦合等离子体发射光谱法（ICP - OES）测定血液、肝脏、胫骨、肾脏和肠壁中的铝浓度。在铝组和铝+柠檬酸盐组中，铝血浓度在1小时和2小时达到峰值，铝+柠檬酸盐组中的水平更高。铝灌胃导致肠壁中铝水平升高，但在其他研究组织中未升高。铝与柠檬酸盐同时灌胃显著增加了其在胫骨、肾脏和肠壁中的组织水平。我们的数据清楚地表明，氯化铝形式的铝可以被吸收，但生物体组织对其保留不佳。此外，本研究中使用的模型适用于急性研究，以研究各种化合物对大鼠铝吸收的影响。
