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钝缘蜱精子包囊的扫描电镜和透射电镜观察:对其运动性的解释尝试

Scanning and transmission electron microscopy of the spermiophores of Ornithodoros ticks: an attempt to explain their motility.

作者信息

Feldman-Muhsam B, Filshie B K

出版信息

Tissue Cell. 1976;8(3):411-9. doi: 10.1016/0040-8166(76)90002-1.

DOI:10.1016/0040-8166(76)90002-1
PMID:982419
Abstract

The spermiophores of two tick species, the kangaroo tick, Ornithodoros gurneyi and the cave tick, Ornithodoros tholozani have been examined by scanning and transmission electron microscopy. The anterior end (head) of the spermiophore is a hemisphere covered with a hexagonal network of small projections. The rest of the spermiophore is covered with longitudinal ridges, seen in sections as cellular processes whose membranes are attached only at their anterior ends by specialized 'feet'. In the cytoplasm of the sperm cell body and just beneath the cellular processes are find filaments, which form a continuous layer in O. tholozani and bundles in O. gurneyi. Fibrils tend to be situated beneath the larger cellular processes. In scanning micrographs helical constrictions have been observed in the posterior parts of some spermiophores. It is proposed that certain of the movements observed by light microscopy in living cultures of spermiophores may be explained by contraction of the cytoplasmic filaments seen in the electron microscope.

摘要

利用扫描电子显微镜和透射电子显微镜对两种蜱类的精包进行了研究,这两种蜱分别是袋鼠蜱(Ornithodoros gurneyi)和洞穴蜱(Ornithodoros tholozani)。精包的前端(头部)是一个半球形,覆盖着由小突起构成的六边形网络。精包的其余部分覆盖着纵向脊,在切片中表现为细胞突起,其膜仅在前端通过特殊的“脚”附着。在精子细胞体的细胞质中以及细胞突起的正下方发现有细丝,这些细丝在洞穴蜱中形成连续层,在袋鼠蜱中形成束状。原纤维往往位于较大的细胞突起下方。在扫描显微照片中,在一些精包的后部观察到螺旋状收缩。有人提出,光学显微镜下在活的精包培养物中观察到的某些运动,可能是由电子显微镜中看到的细胞质细丝收缩所解释的。

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Morphology of the spermatid and spermatozoon of Amblyomma hebraeum Koch (Acarina; Ixodidae).希伯来花蜱(硬蜱科;蜱螨亚纲)精子细胞和精子的形态学
Z Parasitenkd. 1978 Mar 16;55(1):91-9. doi: 10.1007/BF00383479.