Iwaki M, Murakami E, Kikuchi M, Wada A, Ogiso T, Oda Y, Kubo K, Kakehi K
Faculty of Pharmaceutical Sciences, Kinki University, Higashi-osaka, Japan.
J Chromatogr B Biomed Sci Appl. 1998 Sep 25;716(1-2):335-42. doi: 10.1016/s0378-4347(98)00327-2.
Nicotinic acid, nicotinamide and their possible metabolites were successfully separated within 17 min by micellar electrokinetic chromatography using 50 mM borate buffer (pH 9.0) containing 150 mM sodium dodecyl sulfate as the running buffer. Calibration curves for all compounds showed good linearity in a range of 5 microg/ml and 250 microg/ml with good correlation. The present method did not require any clean-up procedures and made it possible to determine all metabolites without interference on a photodiode array detector. Urine samples collected from Wistar male rats were analyzed after high-dose oral or intravenous administration of nicotinic acid or nicotinamide. Metabolic pathways of nicotinic acid in male Wistar rats are also discussed.
采用含150 mM十二烷基硫酸钠的50 mM硼酸盐缓冲液（pH 9.0）作为运行缓冲液，通过胶束电动色谱法在17分钟内成功分离了烟酸、烟酰胺及其可能的代谢产物。所有化合物的校准曲线在5微克/毫升至250微克/毫升范围内显示出良好的线性和相关性。本方法无需任何净化程序，可在光电二极管阵列检测器上无干扰地测定所有代谢产物。对高剂量口服或静脉注射烟酸或烟酰胺后收集的Wistar雄性大鼠尿液样本进行了分析。还讨论了雄性Wistar大鼠中烟酸的代谢途径。
