Sun J, Tadokoro S, Imanaka T, Murakami S D, Nakamura M, Kashiwada K, Ko J, Nishida W, Sobue K
Department of Neurochemistry and Neuropharmacology, Biomedical Research Center, Osaka University Medical School, Suita, Japan.
FEBS Lett. 1998 Oct 23;437(3):304-8. doi: 10.1016/s0014-5793(98)01256-3.
Using monoclonal antibody against the 45 kDa postsynaptic density protein, we isolated a novel isoform of Homer/vesl. The NH2-terminal region containing a PDZ domain of this protein is identical to that of Homer/vesl, and the COOH-terminal region containing unique leucine zippers shows self-multimerization. We named this protein PSD-Zip45. In addition to specific binding of PSD-Zip45 mediated by a PDZ domain to the metabotropic glutamate receptors 1alpha or 5, the distribution of PSD-Zip45 transcripts is highly consistent with that of metabotropic glutamate receptor transcripts. The PSD-Zip45 is, therefore, the first candidate as receptor anchoring proteins containing leucine zipper motifs in the central nervous system.
利用针对45 kDa突触后致密蛋白的单克隆抗体，我们分离出了一种新型的Homer/vesl异构体。该蛋白含PDZ结构域的NH2末端区域与Homer/vesl的相同，而含独特亮氨酸拉链的COOH末端区域表现出自我多聚化。我们将此蛋白命名为PSD-Zip45。除了由PDZ结构域介导的PSD-Zip45与代谢型谷氨酸受体1α或5的特异性结合外，PSD-Zip45转录本的分布与代谢型谷氨酸受体转录本的分布高度一致。因此，PSD-Zip45是中枢神经系统中首个作为含亮氨酸拉链基序的受体锚定蛋白的候选物。
