人胚胎及胎儿肠道中的黏蛋白基因表达。
Mucin gene expression in human embryonic and fetal intestine.
作者信息
Buisine M P, Devisme L, Savidge T C, Gespach C, Gosselin B, Porchet N, Aubert J P
机构信息
Unité 377 INSERM and Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital C Huriez, 59045 Lille Cedex, France.
出版信息
Gut. 1998 Oct;43(4):519-24. doi: 10.1136/gut.43.4.519.
BACKGROUND
The intestinal epithelium is covered by a continuous layer of mucus which is secreted by well differentiated epithelial cells. Disregulation of the expression of mucins has been reported to have possible implications in the neoplastic process which affects intestinal mucosae. It is well known that preneoplastic and neoplastic tissues can express fetal phenotypic characteristics.
AIMS
To assess whether the expression of mucin genes in the intestinal tract is linked to the stage of cellular differentiation and tissue development, by studying the expression of six mucin genes in human fetal small intestine and colon, and also adult tissues.
METHODS
In situ hybridisation was used to study mRNA expression of MUC2, MUC3, MUC4, MUC5B, MUC5AC, and MUC6 in 32 human embryos and fetuses (6.5-27 weeks gestation). Normal adult mucosae were used as controls.
RESULTS
Three mucin genes, MUC2, MUC4, and MUC5AC, were differently expressed in fetal intestine compared with expression in normal adults.
CONCLUSION
These differences in mucin gene expression suggest a possible regulatory role for these products in intestinal epithelial cell differentiation.
背景
肠道上皮由一层连续的黏液覆盖，该黏液由高度分化的上皮细胞分泌。据报道，黏蛋白表达失调可能在影响肠道黏膜的肿瘤形成过程中发挥作用。众所周知，癌前组织和肿瘤组织可表达胎儿表型特征。
目的
通过研究6种黏蛋白基因在人胎儿小肠和结肠以及成人组织中的表达，评估肠道中黏蛋白基因的表达是否与细胞分化阶段和组织发育相关。
方法
采用原位杂交技术研究32例人胚胎和胎儿（妊娠6.5 - 27周）中MUC2、MUC3、MUC4、MUC5B、MUC5AC和MUC6的mRNA表达。以正常成人黏膜作为对照。
结果
与正常成人相比，胎儿肠道中3种黏蛋白基因MUC2、MUC4和MUC5AC表达不同。
结论
黏蛋白基因表达的这些差异表明这些产物可能在肠道上皮细胞分化中起调节作用。
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