碱性成纤维细胞生长因子在完整及溃疡的人胃黏膜中的表达
Expression of basic fibroblast growth factor in intact and ulcerated human gastric mucosa.
作者信息
Hull M A, Brough J L, Powe D G, Carter G I, Jenkins D, Hawkey C J
机构信息
Division of Gastroenterology, University Hospital, Nottingham, UK.
出版信息
Gut. 1998 Oct;43(4):525-36. doi: 10.1136/gut.43.4.525.
BACKGROUND
Basic fibroblast growth factor (bFGF) promotes angiogenesis and healing of gastric ulcers in rats, and bFGF expression is up regulated in such ulcers. However, little is known about expression of bFGF in human gastric mucosa.
AIMS
To investigate bFGF expression in intact human gastric mucosa and gastric ulcers and to determine whether low bFGF content or altered binding by mucosa is associated with ulceration.
SUBJECTS
Endoscopy outpatients, gastrectomy patients, and organ donors.
METHODS
bFGF was isolated by heparin affinity chromatography and characterised by western blotting and endothelial cell bioassay. bFGF was measured by immunoassay and its distribution defined by immunohistochemistry and in situ hybridisation. Binding of bFGF by heparan sulphate proteoglycans was investigated by sodium chloride and heparin extraction.
RESULTS
Bioactive bFGF (19 kDa) was detected in normal mucosa but bFGF mRNA was not found. bFGF expression was up regulated in granulation tissue endothelial cells, mononuclear cells, and epithelial cells at the ulcer rim. Gastric ulcer patients had constitutively low bFGF concentrations in intact antral mucosa which were not explained by changes in binding to heparan sulphate proteoglycans.
CONCLUSIONS
bFGF expression is up regulated in human gastric ulcers. Low intact mucosal bFGF content is associated with gastric ulceration.
背景
碱性成纤维细胞生长因子（bFGF）可促进大鼠胃溃疡的血管生成和愈合，且在这类溃疡中bFGF表达上调。然而，关于bFGF在人胃黏膜中的表达情况却知之甚少。
目的
研究bFGF在完整人胃黏膜和胃溃疡中的表达，并确定低bFGF含量或黏膜结合改变是否与溃疡形成有关。
对象
内镜门诊患者、胃切除患者及器官捐献者。
方法
通过肝素亲和层析法分离bFGF，并采用蛋白质印迹法和内皮细胞生物测定法进行鉴定。通过免疫测定法检测bFGF，并采用免疫组织化学和原位杂交法确定其分布。通过氯化钠和肝素提取法研究硫酸乙酰肝素蛋白聚糖对bFGF的结合情况。
结果
在正常黏膜中检测到生物活性bFGF（19 kDa），但未发现bFGF mRNA。在肉芽组织内皮细胞、单核细胞及溃疡边缘的上皮细胞中bFGF表达上调。胃溃疡患者完整胃窦黏膜中bFGF浓度持续较低，这无法用硫酸乙酰肝素蛋白聚糖结合的变化来解释。
结论
人胃溃疡中bFGF表达上调。完整黏膜中低bFGF含量与胃溃疡形成有关。
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